Ultrastructure and Function of the Stalk Gland Complex of Pompholyx faciemlarva (Rotifera: Monogononta)

Abstract

Many planktonic rotifers carry their oviposited eggs until hatching. In some species, the eggs are attached to the mother via secretions from her style gland, which forms a thread that extends from her cloaca. In species of Pompholyx, the mother possesses the rare ability to change the tension on the secreted thread, which alters the proximity of the egg with respect to her body. In this study, we used behavioral observations, confocal microscopy, and transmission electron microscopy to study the functional morphology of the stalk gland, which secretes a similar thread to the style gland. Our observations reveal that six longitudinal muscles insert on a stalk-gland complex, which is a combination of a two-headed gland and an epithelial duct that connects to the posterior cloaca. The gland secretes a single, long, electron-dense thread that traverses the duct and attaches to the egg surface through the cloaca. Three retractor muscles insert on the stalk gland and function to pull the entire complex anteriorly, thereby increasing tension on the thread and moving the egg close to the mother's body. A set of three (two pairs and a single dorsal) protractor muscles antagonize these actions, and their contraction pulls the gland complex close to the cloaca, thereby releasing tension on the thread and allowing the egg to distance itself from the mother. The stalk gland complex does not appear to be homologous to the style glands of other rotifers, but we hypothesize that it functions as a form of maternal protection as is the case with style glands.