Molecular characterization of Staphylococcus aureus from nasal samples of healthy pet cats.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Letters in Applied Microbiology Pub Date : 2024-11-06 DOI:10.1093/lambio/ovae108

Amel Bezzi, Kenza Antri, Mohamed Azzedine Bachtarzi, Patricia Martins-Simoes, Benjamin Youenou, Samir Gourari, Farida Nateche, Anne Tristan

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Abstract

The objective of this study was to characterize Staphylococcus aureus isolates recovered from the nasal samples of healthy pet cats in Algiers province. A total of 138 nasal swabs were collected. Antimicrobial susceptibility was conducted using the disk-diffusion method and the VITEK-2 susceptibility system. Whole genome sequencing was performed to identify multiple-locus sequence typing, antimicrobial and virulence genes. Staphylococcus aureus isolates were detected in 23 cats. Among these, 11 were methicillin-resistant S. aureus (MRSA) (one isolate/sample). Three sequence types (ST6, ST5, and ST1) were identified in MRSA, with the predominance of ST6 (n = 7). Seven distinct STs [ST398, ST97, ST15, ST7, ST291, ST5043, and a new ST, (ST9219)] were detected in methicillin-sensitive S. aureus. All MRSA isolates harbored the mecA gene and SCCmec-type-IVa. MRSA exhibited resistance to tetracycline [n = 3/tet(L) and tet(M); n = 1/tet(K)], kanamycin-tobramycin [n = 3/ant(4')-Ia), amikacin-kanamycin (n = 1/aph(3')-IIIa], and erythromycin-clindamycin [n = 1/erm(C)]. Seven S. aureus isolates were multidrug resistant. All the isolates were negative for lukS/lukF-PV and tst-1 genes, while 20 isolates were IEC-positive. This study revealed a diversity of genetic lineages in S. aureus strains isolated from nasal samples of pet cats, including multidrug-resistant and toxigenic strains. The presence of IEC-positive S. aureus suggests possible human-animal transmission.

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健康宠物猫鼻腔样本中金黄色葡萄球菌的分子特征。

本研究旨在分析从阿尔及尔省健康宠物猫鼻腔样本中分离出的金黄色葡萄球菌（S. aureus）的特征。共采集了 138 份鼻拭子。采用盘扩散法和 VITEK-2 药敏系统进行抗菌药物药敏试验。进行了全基因组测序（WGS），以确定多焦点序列分型（MLST）、抗菌基因和毒力基因。在 23 只猫中检测到了金黄色葡萄球菌分离物。其中 11 例为耐甲氧西林金黄色葡萄球菌（MRSA）（1 例/样本）。在 MRSA 中发现了三种序列类型（ST6、ST5 和 ST1），其中以 ST6 型为主（n = 7）。在对甲氧西林敏感的金黄色葡萄球菌（MSSA）中检测到七种不同的 ST（ST398、ST97、ST15、ST7、ST291、ST5043 和一种新的 ST（ST9219））。所有 MRSA 分离物都携带 mecA 基因和 SCCmec-type-IVa 基因。MRSA 对四环素（n = 3/tet(L) 和 tet(M)；n = 1/tet(K)）、卡那霉素-妥布霉素（n = 3/ant(4')-Ia）、阿米卡星-卡那霉素（n = 1/aph(3')-IIIa）和红霉素-林可霉素（n = 1/erm(C)）具有耐药性。7 个金黄色葡萄球菌分离株具有多重耐药性。所有分离菌株的 lukS/lukF-PV 和 tst-1 基因均为阴性，而 20 个分离菌株的 IEC 为阳性。这项研究揭示了从宠物猫鼻腔样本中分离出的金黄色葡萄球菌菌株基因谱系的多样性，包括耐多药菌株和致毒菌株。IEC 阳性金黄色葡萄球菌的出现表明可能存在人兽传播。

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来源期刊

Letters in Applied Microbiology 工程技术-生物工程与应用微生物

CiteScore

4.40

自引率

4.20%

发文量

225

审稿时长

3.3 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.