Capsaicin Exerts Antitumor Activity in Mesothelioma Cells.

IF 4.8 2区医学 Q1 NUTRITION & DIETETICS Nutrients Pub Date : 2024-11-01 DOI:10.3390/nu16213758

Emanuela Andretta, Aurora Costa, Elisa Ventura, Massimiliano Quintiliani, Sara Damiano, Antonio Giordano, Andrea Morrione, Roberto Ciarcia

{"title":"Capsaicin Exerts Antitumor Activity in Mesothelioma Cells.","authors":"Emanuela Andretta, Aurora Costa, Elisa Ventura, Massimiliano Quintiliani, Sara Damiano, Antonio Giordano, Andrea Morrione, Roberto Ciarcia","doi":"10.3390/nu16213758","DOIUrl":null,"url":null,"abstract":"Background/objectives: Mesothelioma is an aggressive cancer with limited treatment options. Mesothelioma therapy often involves a multimodal approach including surgery, radiotherapy and chemotherapy. However, the prognosis for patients remains poor. Difficult diagnosis, late symptoms when the tumor is in an advanced stage and the onset of chemotherapy resistance make mesothelioma difficult to treat. For this reason, it is essential to discover new pharmacological approaches. Capsaicin (CAPS) is the active compound of chili peppers. Based on CAPS's anticancer properties on various tumor lines and its chemo-sensitizing action on resistant cells, in this study, we evaluated the effects of CAPS on mesothelioma cells to assess its potential use in mesothelioma therapy.Methods: To evaluate antiproliferative effects of CAPS, we performed MTS assays on various mesothelioma cells, representative of all major mesothelioma subtypes. Transwell migration and wound-healing assays were used to examine the effect of CAPS on mesothelioma cell migration. We also determined the effects of CAPS on oncogenic signaling pathways by assessing the levels of AKT and MAPK activation.Results: In this study, we show that CAPS significantly reduces proliferation of both parental and cisplatin-resistant mesothelioma cells. CAPS promotes S-phase cell cycle arrest and inhibits lateral motility and migration of mesothelioma cells. Accordingly, CAPS suppresses AKT and ERK1/2 activation in MSTO-211H and NCI-H2052 cells. Our results support an antitumor effect of CAPS on cisplatin-resistant mesothelioma cells, suggesting that it may reduce resistance to cisplatin.Conclusions: Our results could pave the way for further studies to evaluate the use of CAPS for mesothelioma treatment.","PeriodicalId":19486,"journal":{"name":"Nutrients","volume":"16 21","pages":""},"PeriodicalIF":4.8000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547426/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nutrients","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/nu16213758","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}

引用次数: 0

Abstract

Background/objectives: Mesothelioma is an aggressive cancer with limited treatment options. Mesothelioma therapy often involves a multimodal approach including surgery, radiotherapy and chemotherapy. However, the prognosis for patients remains poor. Difficult diagnosis, late symptoms when the tumor is in an advanced stage and the onset of chemotherapy resistance make mesothelioma difficult to treat. For this reason, it is essential to discover new pharmacological approaches. Capsaicin (CAPS) is the active compound of chili peppers. Based on CAPS's anticancer properties on various tumor lines and its chemo-sensitizing action on resistant cells, in this study, we evaluated the effects of CAPS on mesothelioma cells to assess its potential use in mesothelioma therapy.

Methods: To evaluate antiproliferative effects of CAPS, we performed MTS assays on various mesothelioma cells, representative of all major mesothelioma subtypes. Transwell migration and wound-healing assays were used to examine the effect of CAPS on mesothelioma cell migration. We also determined the effects of CAPS on oncogenic signaling pathways by assessing the levels of AKT and MAPK activation.

Results: In this study, we show that CAPS significantly reduces proliferation of both parental and cisplatin-resistant mesothelioma cells. CAPS promotes S-phase cell cycle arrest and inhibits lateral motility and migration of mesothelioma cells. Accordingly, CAPS suppresses AKT and ERK1/2 activation in MSTO-211H and NCI-H2052 cells. Our results support an antitumor effect of CAPS on cisplatin-resistant mesothelioma cells, suggesting that it may reduce resistance to cisplatin.

Conclusions: Our results could pave the way for further studies to evaluate the use of CAPS for mesothelioma treatment.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

辣椒素对间皮瘤细胞具有抗肿瘤活性

背景/目的：间皮瘤是一种侵袭性癌症，治疗方法有限。间皮瘤治疗通常采用多模式方法，包括手术、放疗和化疗。然而，患者的预后仍然很差。诊断困难、肿瘤晚期症状出现较晚以及化疗耐药性的出现，都使间皮瘤难以治疗。因此，发现新的药物治疗方法至关重要。辣椒素（CAPS）是辣椒的活性化合物。基于辣椒素对多种肿瘤细胞株的抗癌特性及其对耐药细胞的化疗增敏作用，我们在本研究中评估了辣椒素对间皮瘤细胞的影响，以评估其在间皮瘤治疗中的潜在用途：为了评估 CAPS 的抗增殖作用，我们对各种间皮瘤细胞进行了 MTS 检测，这些细胞代表了所有主要的间皮瘤亚型。透孔迁移和伤口愈合试验用于检测 CAPS 对间皮瘤细胞迁移的影响。我们还通过评估 AKT 和 MAPK 的激活水平，确定了 CAPS 对致癌信号通路的影响：在这项研究中，我们发现 CAPS 能显著减少亲代和顺铂耐药间皮瘤细胞的增殖。CAPS 可促进 S 期细胞周期的停滞，抑制间皮瘤细胞的横向移动和迁移。因此，CAPS抑制了MSTO-211H和NCI-H2052细胞中AKT和ERK1/2的活化。我们的研究结果支持 CAPS 对顺铂耐药间皮瘤细胞的抗肿瘤作用，表明它可能会降低对顺铂的耐药性：我们的研究结果可为进一步研究评估 CAPS 在间皮瘤治疗中的应用铺平道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Nutrients NUTRITION & DIETETICS-

CiteScore

9.20

自引率

15.30%

发文量

4599

审稿时长

16.74 days

期刊介绍： Nutrients (ISSN 2072-6643) is an international, peer-reviewed open access advanced forum for studies related to Human Nutrition. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.