{"title":"The Hcp2b of APEC induces mitochondrial damage in chicken DF-1 cells.","authors":"Liting Lu, Zhao Qi, Haiyang Wang, Zhe Chen, Zichao Song, Ziqi Li, Xiaoru Wang, Bingyu Zhao, Xiyang Wei, Ying Shao, Zhenyu Wang, Jian Tu, Xiangjun Song","doi":"10.1080/03079457.2024.2431803","DOIUrl":null,"url":null,"abstract":"<p><p>The haemolysin co-regulatory protein (Hcp) plays a significant role in the pathogenicity of avian pathogenic <i>Escherichia coli</i> (APEC) as an effector protein of the type VI secretion system (T6SS) to the host. Meanwhile, mitochondria in the host are the target of effector proteins of various secretion systems. Here, we explored the effects of APEC effector Hcp2b on the mitochondria of DF-1 cells and found that Hcp2b results in damage in mitochondria. Next, 68 target proteins in DF-1 cell lysates were identified that interacted with Hcp2b by streptavidin-biotin pull-down assay combined with LC-MS/MS, among which ADP/ATP transporter carrier (SLC25A4) is a mitochondria-associated protein; protein docking analysis showed that Hcp2b binds well to SLC25A4. Therefore, we hypothesize that the Hcp2b contributes to mitochondrial damage in DF-1 cells through interaction with the SLC25A4.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5000,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avian Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/03079457.2024.2431803","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The haemolysin co-regulatory protein (Hcp) plays a significant role in the pathogenicity of avian pathogenic Escherichia coli (APEC) as an effector protein of the type VI secretion system (T6SS) to the host. Meanwhile, mitochondria in the host are the target of effector proteins of various secretion systems. Here, we explored the effects of APEC effector Hcp2b on the mitochondria of DF-1 cells and found that Hcp2b results in damage in mitochondria. Next, 68 target proteins in DF-1 cell lysates were identified that interacted with Hcp2b by streptavidin-biotin pull-down assay combined with LC-MS/MS, among which ADP/ATP transporter carrier (SLC25A4) is a mitochondria-associated protein; protein docking analysis showed that Hcp2b binds well to SLC25A4. Therefore, we hypothesize that the Hcp2b contributes to mitochondrial damage in DF-1 cells through interaction with the SLC25A4.
期刊介绍：
Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.
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