Homogeneous synthesis of cationic celluloses with broad-spectrum antibacterial activities for the treatment of vaginitis in mice

Abstract

Microbial infection is a significant health issue for humans. Despite the development of numerous antibiotics, the continuous rise of drug-resistant bacteria highlights the urgent need for new materials to combat these problems. In this study, four water-soluble quaternized cellulose (QC) derivatives with degrees of substitution (DS) ranging from 0.23 to 0.45 were synthesized homogeneously from cellulose carbamate (CC) in NaOH/ZnO aqueous solution. The QC derivatives exhibited broad-spectrum antibacterial activity against gram-negative/positive bacteria, fungi and drug-resistance bacteria. Models of bacterial vaginitis (BV) and vulvovaginal candidiasis (VVC) were used to evaluate the application of QC derivatives visually. Secretion smears and tissue section staining revealed that treatment with QC derivatives led to a reduction in mycelia and spores in the vagina and secretions in the VVC model, along with improved inflammation. In the BV model, vaginal secretions were reduced, clue cells in smears significantly decreased, and inflammation markedly improved. Additionally, cell experiments and staining of mouse organ tissue sections demonstrated that QC derivatives exhibited good biocompatibility. Therefore, using QC derivatives in flushing douches represents a novel approach for treating vaginitis and could serve as a benchmark for addressing other infectious diseases.