Screening, characterization, and production of Bacillus cereus (S55) bioflocculant isolated from soil for application in wastewater treatment

Abstract

This research evaluated a bacterial strain, Bacillus cereus (S55), isolated from the soil for its bioflocculant production capabilities. The strain was identified through morphological and 16s rRNA gene sequencing. The optimization of culture conditions, using One-factor-at-a-time method, significantly enhanced bioflocculant production with glucose and urea. FTIR analysis showed the presence of hydroxyl, amine and carboxylate functional groups, with polysaccharides as predominant components. Scanning electron microscopy and X-ray diffraction confirmed the crystalline nature of the bioflocculant. The strain studied showed potential in treating household wastewater and was effective at removing dyes, suggesting alternatives for wastewater management.