A novel label free electrochemical aptasensor based on poly(anthranilic acid-co-aniline) for detection of miRNA-155, a cancer biomarker

IF 4.1 3区工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC Sensors and Actuators A-physical Pub Date : 2024-11-12 DOI:10.1016/j.sna.2024.116053

Zahra Aghaei , Ali Ghaffarinejad

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Abstract

Traditional electrochemical biosensors often rely on expensive materials like gold nanoparticles within their electrode structures. In contrast to existing methods, this research introduces a novel, groundbreaking, label-free aptasensor. This sensor comprises simple and inexpensive materials, making it accessible and budget-conscious. It boasts exceptional sensitivity, allowing for the identification of leukemia cancer biomarker (miRNA-155) in its early stages. The proposed aptasensor consisted of a copolymer of poly anthranilic acid and polyaniline on the graphite sheet, in which the receptor of miRNA-155 was self-assembled on the copolymer. The substrate of receptors has benefits, including high electrical conductivity, excellent stability, good biocompatibility, and simple synthesis. The important variables affecting the adequacy of the designed aptasensor were examined in the presence of [Fe(CN)₆]^3-/4- as a redox probe. The proposed aptasensor was successfully used in human blood plasma. Due to the ease of preparation, cost, and time effectiveness, the proposed aptasensor is promising for mass production. Finally, at optimum conditions, the sensor exhibited a linear response within a concentration range of 0.1 nM to 1 aM with a detection limit of 1 aM.

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基于聚(蒽酸-苯胺)的新型无标记电化学适配器，用于检测癌症生物标记物 miRNA-155

传统的电化学生物传感器通常依赖于昂贵的材料，如电极结构中的金纳米粒子。与现有方法不同的是，这项研究引入了一种新颖、突破性的无标记传感器。这种传感器由简单而廉价的材料组成，使其易于使用并具有预算意识。它具有极高的灵敏度，可在早期阶段识别白血病癌症生物标志物（miRNA-155）。拟议的灵敏传感器由石墨片上的聚蒽酸和聚苯胺共聚物组成，其中 miRNA-155 的受体自组装在共聚物上。受体基质具有导电性强、稳定性好、生物相容性好、合成简单等优点。在[Fe(CN)6]3-/4-作为氧化还原探针的情况下，研究了影响所设计的适配传感器适当性的重要变量。所提出的适配传感器成功地应用于人体血浆中。由于制备简单、成本低、时间短，该传感器有望大规模生产。最后，在最佳条件下，传感器在 0.1 nM 至 1 aM 的浓度范围内呈线性响应，检测限为 1 aM。

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来源期刊

Sensors and Actuators A-physical 工程技术-工程：电子与电气

CiteScore

8.10

自引率

6.50%

发文量

630

审稿时长

49 days

期刊介绍： Sensors and Actuators A: Physical brings together multidisciplinary interests in one journal entirely devoted to disseminating information on all aspects of research and development of solid-state devices for transducing physical signals. Sensors and Actuators A: Physical regularly publishes original papers, letters to the Editors and from time to time invited review articles within the following device areas: • Fundamentals and Physics, such as: classification of effects, physical effects, measurement theory, modelling of sensors, measurement standards, measurement errors, units and constants, time and frequency measurement. Modeling papers should bring new modeling techniques to the field and be supported by experimental results. • Materials and their Processing, such as: piezoelectric materials, polymers, metal oxides, III-V and II-VI semiconductors, thick and thin films, optical glass fibres, amorphous, polycrystalline and monocrystalline silicon. • Optoelectronic sensors, such as: photovoltaic diodes, photoconductors, photodiodes, phototransistors, positron-sensitive photodetectors, optoisolators, photodiode arrays, charge-coupled devices, light-emitting diodes, injection lasers and liquid-crystal displays. • Mechanical sensors, such as: metallic, thin-film and semiconductor strain gauges, diffused silicon pressure sensors, silicon accelerometers, solid-state displacement transducers, piezo junction devices, piezoelectric field-effect transducers (PiFETs), tunnel-diode strain sensors, surface acoustic wave devices, silicon micromechanical switches, solid-state flow meters and electronic flow controllers. Etc...