Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng.

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Genomics Pub Date : 2024-11-18 DOI:10.1186/s12864-024-10989-6

Yuping Xiong, Xiaohong Chen, Junyu Liu, Yuan Li, Zhan Bian, Xinhua Zhang, Songjun Zeng, Jaime A Teixeira da Silva, Guohua Ma

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Abstract

Background: The extract from Metasequoia glyptostroboides Hu et Cheng, a rare and endangered species native to China, exhibits numerous biological and pharmacological activities. The species is recalcitrant to rooting during micropropagation, a challenge that has yet to be resolved. In this study, transcriptomic and hormonal analyses were conducted to appreciate the molecular mechanism of adventitious root (AR) formation in optimized rooting conditions.

Results: The use of 2/5-strength Woody Plant Medium (WPM) significantly promoted AR formation of M. glyptostroboides shoots while the content of endogenous auxin, cytokinins and gibberellins (GAs) varied at different stages of AR formation. Transcriptomic analysis showed the significant up- or down-regulation of differentially expressed genes (DEGs) associated with plant hormone signal transduction and the phenylpropanoid biosynthesis pathway in response to 2/5-strength WPM. DEGs related to the biosynthesis of indole-3-acetic acid, cytokinins and GAs were identified. Transcript factors involved in 13 families were also revealed. A weighted gene co-expression network analysis indicated a strong correlation between hormones and genes involved in plant hormone signal transduction and the phenylpropanoid biosynthetic pathway.

Conclusions: These results indicate that the AR-promoting potential of 2/5-strength WPM in M. glyptostroboides was due to complex interactions between hormones and the expression of genes related to plant hormone signal transduction and the phenylpropanoid biosynthetic pathway.

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转录组和激素的比较分析揭示了水杉不定根形成的潜在调控网络 Hu et Cheng.

研究背景水杉的提取物具有多种生物和药理活性。该物种在微繁殖过程中难以生根，这一难题至今尚未解决。本研究通过转录组和激素分析，了解在优化生根条件下不定根（AR）形成的分子机制：结果：使用 2/5 浓度的木本植物培养基（WPM）能显著促进草甘膦芽的不定根形成，而在不定根形成的不同阶段，内源辅助素、细胞分裂素和赤霉素（GAs）的含量各不相同。转录组分析表明，与植物激素信号转导和苯丙醇类生物合成途径相关的差异表达基因（DEGs）在2/5强度的WPM作用下有明显的上调或下调。确定了与吲哚-3-乙酸、细胞分裂素和雌酮生物合成有关的 DEGs。还发现了 13 个家族中的转录因子。加权基因共表达网络分析表明，激素与参与植物激素信号转导和苯丙素生物合成途径的基因之间存在很强的相关性：这些结果表明，2/5-Strength WPM 在甘蓝型茎叶中的 AR 促进潜力是由激素与植物激素信号转导和苯丙类生物合成途径相关基因的表达之间复杂的相互作用造成的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Genomics 生物-生物工程与应用微生物

CiteScore

7.40

自引率

4.50%

发文量

769

审稿时长

6.4 months

期刊介绍： BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.