{"title":"Optimal Design, Characterization, and Structure-Function Analysis of Two Variants of Cathelicidin-2","authors":"Zhengyu Liu, Hongfei Wang, Jiali Cai, Bo Yao","doi":"10.1134/S1070363224100141","DOIUrl":null,"url":null,"abstract":"<p>Two variants of cathelicidin-2 were designed and their antimicrobial activity and structure–function relationship were evaluated and discussed. The MICs of LH22 and VL22 against <i>Aeromonas hydrophila</i> were < 0.98 and 15.6 μg/mL, respectively, and the MICs of VL22 against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> were 15.6 and 31.25 μg/mL, respectively. Large amounts of nucleic acids and proteins were detected in the supernatant of culture medium after treatment with LH22 and VL22. Apparent wrinkled and distorted cell morphology and apparent pores could be observed by SEM in the treated <i>Aeromonas hydrophila</i> with the two peptides. These two peptides also showed the neutralizing activity against LPS.</p>","PeriodicalId":761,"journal":{"name":"Russian Journal of General Chemistry","volume":"94 10","pages":"2689 - 2699"},"PeriodicalIF":0.9000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of General Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1134/S1070363224100141","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Two variants of cathelicidin-2 were designed and their antimicrobial activity and structure–function relationship were evaluated and discussed. The MICs of LH22 and VL22 against Aeromonas hydrophila were < 0.98 and 15.6 μg/mL, respectively, and the MICs of VL22 against Staphylococcus aureus and Escherichia coli were 15.6 and 31.25 μg/mL, respectively. Large amounts of nucleic acids and proteins were detected in the supernatant of culture medium after treatment with LH22 and VL22. Apparent wrinkled and distorted cell morphology and apparent pores could be observed by SEM in the treated Aeromonas hydrophila with the two peptides. These two peptides also showed the neutralizing activity against LPS.
期刊介绍：
Russian Journal of General Chemistry is a journal that covers many problems that are of general interest to the whole community of chemists. The journal is the successor to Russia’s first chemical journal, Zhurnal Russkogo Khimicheskogo Obshchestva (Journal of the Russian Chemical Society ) founded in 1869 to cover all aspects of chemistry. Now the journal is focused on the interdisciplinary areas of chemistry (organometallics, organometalloids, organoinorganic complexes, mechanochemistry, nanochemistry, etc.), new achievements and long-term results in the field. The journal publishes reviews, current scientific papers, letters to the editor, and discussion papers.
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