Toll-like receptor-4 expression and oxidative stress in ocular rosacea.

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Vision Pub Date : 2024-03-30 eCollection Date: 2024-01-01

Nilufer Yesilirmak, Neslihan Bukan, Busra Kurt, Tugba Fatsa, Sema Yuzbasıoglu, Min Zhao, Tugrul Hosbul, Jean-Louis Bourges, Francine Behar-Cohen

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Abstract

Purpose: To investigate systemic and ocular toll-like receptor (TLR)-4 expression and its association with oxidative stress markers in ocular rosacea (OR).

Methods: This prospective study included 40 patients with rosacea with ocular involvement and 20 healthy volunteers. Tear break-up time (TBUT), Schirmer test, meibomoscore, and ocular surface disease index (OSDI) scores were estimated for all participants. TLR-4 expression in conjunctival epithelium and peripheral blood mononuclear cells was quantified using real-time polymerase chain reaction (RT-PCR). In the tears and serum samples of all participants, antioxidant status (TAS), total oxidant status (TOS), and arylesterase (ARE) activation levels were measured using a fully automated spectrophotometric method, and the oxidative stress index (OSI) was calculated.

Results: TLR-4 expression levels and oxidative stress status (TOS and OSI values) were significantly higher (p < 0.01), and antioxidant status (TAS and ARE values) were significantly lower (p < 0.01) in both ocular and blood samples of patients with OR compared with those in controls. A significant positive correlation was found between the ocular and blood values in all parameters (p < 0.05). According to the clinical associations of these results, we found negative correlations between TLR-4, OSI, and TBUT and between TLR-4 and Schirmer, whereas a positive correlation was observed between TLR-4, OSI, and meiboscore and between TLR-4, OSI, and OSDI (p < 0.05). No correlation was found between the OSI and Schirmer results (p = 0.92).

Conclusions: TLR-4 and oxidative stress both play important roles in OR pathophysiology and are closely related to clinical findings.

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眼部红斑痤疮中 Toll 样受体-4 的表达和氧化应激。

目的：研究眼部红斑痤疮（OR）中全身和眼部toll样受体（TLR）-4的表达及其与氧化应激标记物的关系：这项前瞻性研究包括 40 名眼部受累的酒渣鼻患者和 20 名健康志愿者。对所有参与者的泪液破裂时间（TBUT）、施尔默试验、meibomoscore和眼表疾病指数（OSDI）评分进行了估计。采用实时聚合酶链反应（RT-PCR）对结膜上皮细胞和外周血单核细胞中的 TLR-4 表达进行了量化。采用全自动分光光度法测量了所有参与者泪液和血清样本中的抗氧化状态（TAS）、总氧化状态（TOS）和芳酯酶（ARE）活化水平，并计算了氧化应激指数（OSI）：结果：与对照组相比，OR 患者眼部和血液样本中的 TLR-4 表达水平和氧化应激状态（TOS 值和 OSI 值）显著升高（p < 0.01），而抗氧化状态（TAS 值和 ARE 值）显著降低（p < 0.01）。所有参数的眼部和血液值之间均存在明显的正相关性（p < 0.05）。根据这些结果的临床关联，我们发现 TLR-4、OSI 和 TBUT 之间以及 TLR-4 和 Schirmer 之间存在负相关，而 TLR-4、OSI 和 meiboscore 之间以及 TLR-4、OSI 和 OSDI 之间存在正相关（p < 0.05）。OSI 和 Schirmer 结果之间没有相关性（p = 0.92）：结论：TLR-4和氧化应激在OR病理生理学中都起着重要作用，并且与临床结果密切相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.