Constitutive expression of cucumber CsACS2 in Arabidopsis Thaliana disrupts anther dehiscence through ethylene signaling and DNA methylation pathways.

IF 5.3 2区生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-11-21 DOI:10.1007/s00299-024-03374-w

Zonghui Yang, Libin Li, Zhaojuan Meng, Mingqi Wang, Tian Gao, Jingjuan Li, Lixia Zhu, Qiwei Cao

{"title":"Constitutive expression of cucumber CsACS2 in Arabidopsis Thaliana disrupts anther dehiscence through ethylene signaling and DNA methylation pathways.","authors":"Zonghui Yang, Libin Li, Zhaojuan Meng, Mingqi Wang, Tian Gao, Jingjuan Li, Lixia Zhu, Qiwei Cao","doi":"10.1007/s00299-024-03374-w","DOIUrl":null,"url":null,"abstract":"<p><strong>Key message: </strong>Constitutive expression of cucumber CsACS2 in Arabidopsis disrupts anther dehiscence and male fertility via ethylene signaling and DNA methylation, revealing new avenues for enhancing crop reproductive traits. The cucumber gene CsACS2, encoding ACC (1-aminocyclopropane-1-carboxylic acid) synthase, plays a pivotal role in ethylene biosynthesis and sex determination. This study investigates the effects of constitutive CsACS2 expression in Arabidopsis thaliana on anther development and male fertility. Transgenic Arabidopsis plants overexpressing CsACS2 exhibited male sterility due to inhibited anther dehiscence, which was linked to suppressed secondary cell wall thickening. RNA-Seq analysis revealed upregulation of ethylene signaling pathway genes and downregulation of secondary cell wall biosynthesis genes, with gene set enrichment analysis indicating the involvement of DNA methylation. Rescue experiments demonstrated that silver nitrate (AgNO₃) effectively restored fertility, while 5-azacytidine (5-az) partially restored it, highlighting the roles of ethylene signaling and DNA methylation in this process. Constitutive CsACS2 expression in Arabidopsis disrupts anther development through ethylene signaling and DNA methylation pathways, providing new insights into the role of ethylene in plant reproductive development and potential applications in crop improvement.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"288"},"PeriodicalIF":5.3000,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell Reports","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00299-024-03374-w","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Key message: Constitutive expression of cucumber CsACS2 in Arabidopsis disrupts anther dehiscence and male fertility via ethylene signaling and DNA methylation, revealing new avenues for enhancing crop reproductive traits. The cucumber gene CsACS2, encoding ACC (1-aminocyclopropane-1-carboxylic acid) synthase, plays a pivotal role in ethylene biosynthesis and sex determination. This study investigates the effects of constitutive CsACS2 expression in Arabidopsis thaliana on anther development and male fertility. Transgenic Arabidopsis plants overexpressing CsACS2 exhibited male sterility due to inhibited anther dehiscence, which was linked to suppressed secondary cell wall thickening. RNA-Seq analysis revealed upregulation of ethylene signaling pathway genes and downregulation of secondary cell wall biosynthesis genes, with gene set enrichment analysis indicating the involvement of DNA methylation. Rescue experiments demonstrated that silver nitrate (AgNO₃) effectively restored fertility, while 5-azacytidine (5-az) partially restored it, highlighting the roles of ethylene signaling and DNA methylation in this process. Constitutive CsACS2 expression in Arabidopsis disrupts anther development through ethylene signaling and DNA methylation pathways, providing new insights into the role of ethylene in plant reproductive development and potential applications in crop improvement.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

拟南芥中黄瓜 CsACS2 的连续表达可通过乙烯信号和 DNA 甲基化途径破坏花药开裂。

关键信息：拟南芥中黄瓜CsACS2的连续表达通过乙烯信号转导和DNA甲基化破坏了花药开裂和雄性繁殖力，为提高作物生殖性状揭示了新途径。编码 ACC（1-氨基环丙烷-1-羧酸）合成酶的黄瓜基因 CsACS2 在乙烯生物合成和性别决定中起着关键作用。本研究探讨了拟南芥中组成型 CsACS2 表达对花药发育和雄性繁殖力的影响。过表达 CsACS2 的转基因拟南芥植株因花药开裂受抑制而表现出雄性不育，这与次生细胞壁增厚受抑制有关。RNA-Seq 分析表明乙烯信号通路基因上调，次生细胞壁生物合成基因下调，基因组富集分析表明 DNA 甲基化参与其中。拯救实验表明，硝酸银（AgNO₃）能有效恢复生育能力，而5-氮杂胞苷（5-az）则能部分恢复生育能力，这突显了乙烯信号转导和DNA甲基化在这一过程中的作用。拟南芥中 CsACS2 的连续表达通过乙烯信号转导和 DNA 甲基化途径破坏了花药的发育，为乙烯在植物生殖发育中的作用以及在作物改良中的潜在应用提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Plant Cell Reports 生物-植物科学

CiteScore

10.80

自引率

1.60%

发文量

135

审稿时长

3.2 months

期刊介绍： Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.