In vitro antibacterial effect of a nano-zinc oxide eugenol sealer alone and in combination with chitosan, propolis, and nanosilver on Enterococcus faecalis.
Parisa Ghaffari, Habib Zeighami, Mohammad Najdalizade, Leila Eftekhar
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引用次数: 0
Abstract
Background: This study aimed to assess the antibacterial effect of a nano-zinc oxide eugenol (nZOE) sealer alone and in combination with chitosan, propolis, and nanosilver on Enterococcus faecalis.
Materials and methods: In this in vitro, experimental study, nanosilver, chitosan, and propolis with 10wt%, 20wt%, and 60wt% concentrations, respectively, were added to nZOE sealer, and their antibacterial activity against E. faecalis was evaluated by agar diffusion and broth microdilution tests. The diameter of the growth inhibition zones was measured, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated for all materials. Data were analyzed by t-test (alpha = 0.05).
Results: The addition of nanosilver, chitosan, and propolis to nZOE did not change the diameter of growth inhibition zone in agar diffusion test. Propolis and eugenol alone showed the lowest MIC and MBC. Chitosan alone showed the highest MIC and MBC. Furthermore, nZOE showed lower MBC than micro-ZOE (P = 0.000). All groups containing nZOE showed the lowest MIC and MBC values.
Conclusion: The addition of propolis to nZOE can enhance its antibacterial activity against E. faecalis in vitro.
背景：本研究旨在评估纳米氧化锌丁香酚（nZOE）封闭剂单独使用以及与壳聚糖、蜂胶和纳米银结合使用对粪肠球菌的抗菌效果：在这项体外实验研究中，纳米银、壳聚糖和蜂胶的浓度分别为 10wt%、20wt% 和 60wt%，它们被添加到 nZOE 封闭剂中，并通过琼脂扩散和肉汤微稀释试验评估了它们对粪肠球菌的抗菌活性。测量了生长抑制区的直径，并计算了所有材料的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值。数据分析采用 t 检验（α = 0.05）：在 nZOE 中添加纳米银、壳聚糖和蜂胶不会改变琼脂扩散试验中生长抑制区的直径。蜂胶和丁香酚的 MIC 和 MBC 最低。壳聚糖的 MIC 和 MBC 最高。此外，nZOE 的 MBC 低于 micro-ZOE（P = 0.000）。所有含有 nZOE 的组的 MIC 值和 MBC 值都最低：结论：在 nZOE 中添加蜂胶可增强其对粪肠球菌的体外抗菌活性。
期刊介绍：
Dental Research Journal, a publication of Isfahan University of Medical Sciences, is a peer-reviewed online journal with Bimonthly print on demand compilation of issues published. The journal’s full text is available online at http://www.drjjournal.net. The journal allows free access (Open Access) to its contents and permits authors to self-archive final accepted version of the articles on any OAI-compliant institutional / subject-based repository. The journal will cover technical and clinical studies related to health, ethical and social issues in field of Dentistry. Articles with clinical interest and implications will be given preference.
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