Lactylated histone H3K18 as a potential biomarker for the diagnosis and prediction of the severity of pancreatic cancer

IF 2.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL Clinics Pub Date : 2024-11-26 DOI:10.1016/j.clinsp.2024.100544

Jinping Hou , Mingsong Guo , Yongqiong Li , Yijin Liao

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Abstract

Background

Lactylation plays an essential role in pancreatic cancer, but the precise role of lactylated histone in the diagnosis and prognosis of pancreatic cancer remains to be further clarified.

Methods

Twenty-one patients diagnosed with pancreatic cancer were enrolled in this study, and the clinicopathologic characteristics were collected. Lactylation levels of total proteins and histone H3 Lysine-18 (H3K18) of tissues were determined by western blotting and laboratory indicators including serum levels of lactate, Cancer Antigen 19–9 (CA19–9), and Carcinoembryogenic Antigen (CEA) were obtained.

Results

Total protein lactylation was found in both pancreatic cancer tissues and para-carcinoma normal tissues, and was more potent in tumor tissues. H3K18la was also highly expressed tumor tissues. Furthermore, H3K18la protein expression correlated positively with serum lactate (r = 0.774, p < 0.001), CA19–9 (r = 0.744, p < 0.001), and CEA (r = 0.589, p < 0.01). The Area Under the Curve (AUC) of H3K18la for the diagnosis of pancreatic cancer was 0.848 in serum (p < 0.001).

Conclusion

The present findings suggested that H3K18 may be used as a novel potential biomarker for the diagnosis and prognosis of pancreatic cancer patients.

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乳化组蛋白 H3K18 作为诊断和预测胰腺癌严重程度的潜在生物标记物

背景乳酸化在胰腺癌中起着至关重要的作用，但乳酸化组蛋白在胰腺癌诊断和预后中的确切作用仍有待进一步明确。结果在胰腺癌组织和癌旁正常组织中均发现总蛋白乳化和组蛋白 H3 赖氨酸-18（H3K18）乳化，且在肿瘤组织中更强。H3K18la也在肿瘤组织中高表达。此外，H3K18la 蛋白表达与血清乳酸（r = 0.774，p < 0.001）、CA19-9（r = 0.744，p < 0.001）和 CEA（r = 0.589，p < 0.01）呈正相关。结论本研究结果表明，H3K18可作为一种新型的潜在生物标记物用于胰腺癌患者的诊断和预后。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinics 医学-医学：内科

CiteScore

4.10

自引率

3.70%

发文量

129

审稿时长

52 days

期刊介绍： CLINICS is an electronic journal that publishes peer-reviewed articles in continuous flow, of interest to clinicians and researchers in the medical sciences. CLINICS complies with the policies of funding agencies which request or require deposition of the published articles that they fund into publicly available databases. CLINICS supports the position of the International Committee of Medical Journal Editors (ICMJE) on trial registration.