Robustly Injectable Tetra-PEG Hydrogel Sealants for Annulus Fibrosus Repair.

IF 10 2区 医学 Q1 ENGINEERING, BIOMEDICAL Advanced Healthcare Materials Pub Date : 2024-11-24 DOI:10.1002/adhm.202403163
Guoke Tang, Yucai Li, Yi Liu, Lan Lin, Jielin Wang, Xing Wang, Xiaojian Ye
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Abstract

Discectomy serves as the primary therapeutic approach for lumbar disc herniation, but the annular fibrosus defects after discectomy may lead to recurrence of disc herniation. Despite recent advances in bioinspired adhesives to seal the AF defect, the growing popularity of endoscopic discectomy has put forward high requirements for the tissue bioadhesives with rapid injectability, easy operation, and robust tissue adhesion in underwater environments. Herein, a rapidly in situ forming injectable tetra-PEG bioadhesive (ISG) comprising of FDA-approved tetra-armed poly (ethylene glycol) amine (tetra-PEG-NH2) and tetra-armed poly (ethylene glycol) succinimidyl glutarate (tetra-PEG-SG) for the sutureless closure of AF defects, is reported. Relying on quick ammonolysis reaction between N-hydroxysuccinimide (NHS)-ester of tetra-PEG-SG polymer and amine groups of tetra-PEG-NH2 polymer and tissue proteins, the uniform networks are formed within seconds with easy injection, efficient waterproofness, instant tissue adhesion, and durable compliance. The goat lumbar discectomy model was used to assess the effect of ISG hydrogels in vivo. The results reveal that the resultant ISG bioadhesive can effectively maintain the disc height, fuse with the host tissue, ameliorate IVD degeneration, and retain the initial biomechanics. Together, this study provides an efficient strategy of in situ injectable glue for the minimally invasive treatment of AF defects.

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用于瓣膜纤维环修复的强效注射型四聚乙二醇水凝胶密封剂
椎间盘切除术是腰椎间盘突出症的主要治疗方法,但椎间盘切除术后纤维环缺损可能导致椎间盘突出症复发。尽管最近在密封椎间盘纤维环缺损的生物启发粘合剂方面取得了进展,但内窥镜椎间盘切除术的日益普及对组织生物粘合剂在水下环境中的快速注射性、易操作性和强大的组织粘附性提出了很高的要求。本文报道了一种快速原位成型的可注射四聚乙二醇生物粘合剂(ISG),由美国 FDA 批准的四臂聚(乙二醇)胺(tetra-PEG-NH2)和四臂聚(乙二醇)琥珀酰亚胺基戊二酸酯(tetra-PEG-SG)组成,用于房颤缺损的无缝合闭合。依靠四-PEG-SG 聚合物的 N-羟基琥珀酰亚胺(NHS)酯和四-PEG-NH2 聚合物的胺基团与组织蛋白质之间的快速氨解反应,可在数秒内形成均匀的网络,具有注射方便、高效防水、瞬间组织粘附和持久顺应性等特点。研究人员利用山羊腰椎间盘切除术模型来评估 ISG 水凝胶在体内的效果。结果表明,ISG 生物粘合剂能有效保持椎间盘高度,与宿主组织融合,改善 IVD 退化,并保持最初的生物力学特性。总之,这项研究为原位注射胶微创治疗房颤缺损提供了一种有效的策略。
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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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