MXene Bimetallic Coating Synergistic Enhanced Colorimetric-Raman Dual Signal-Based Immunochromatographic Assay for Advancing Detection Performance

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2024-11-26 DOI:10.1021/acs.analchem.4c04234

Youwen Deng, Zhiheng Gao, Zhongqi Lin, Zehao Yang, Mengfang Lin, Zhenlin Xu, Hongtao Lei, Xiangmei Li

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Abstract

Herein, a three-dimensional thin film-like multifunctional MXene bimetallic coating material (Ti₃C₂@Au–Ag) with strong color intensity, high surface-enhanced Raman scattering (SERS) activity, and strong antibody affinity (1.00 × 10⁸ M^–1) was prepared. It was the first time that Ti₃C₂@Au–Ag-based colorimetric-SERS dual-signal immunochromatographic assay (ICA) was developed for the detection of dexamethasone, achieving the limits of detection of 0.0089, 0.14, and 0.084 μg/kg for milk, beef, and pork in colorimetric mode and 0.0015, 0.060, and 0.075 μg/kg in SERS mode. It was up to 200-fold more sensitive than the reported ICAs. The recoveries were 82.0%–112.6%, and the coefficients of variation were 1.4%–13.7%. The Ti₃C₂@Au–Ag-ICA was verified by LC–MS/MS in the application on 30 real samples with a correlation coefficient greater than 0.98. This study can provide efficient theoretical and practical value for the development of a colorimetric-SERS dual-signal immunoassay platform.

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基于 MXene 双金属涂层的增效增强型比色-拉曼双信号免疫层析分析仪，用于提高检测性能

本文制备了一种三维薄膜状多功能MXene双金属涂层材料（Ti3C2@Au-Ag），该材料具有较强的颜色强度、较高的表面增强拉曼散射（SERS）活性和较强的抗体亲和力（1.00 × 108 M-1）。首次开发了基于 Ti3C2@Au-Ag 的比色-SERS 双信号免疫层析（ICA）检测地塞米松的方法，在比色模式下，牛奶、牛肉和猪肉的检出限分别为 0.0089、0.14 和 0.084 μg/kg；在 SERS 模式下，检出限分别为 0.0015、0.060 和 0.075 μg/kg。其灵敏度比已报道的 ICA 高出 200 倍。回收率为 82.0%-112.6%，变异系数为 1.4%-13.7%。Ti3C2@Au-Ag-ICA在30个实际样品中的应用得到了LC-MS/MS的验证，相关系数大于0.98。该研究可为比色-SERS双信号免疫分析平台的开发提供有效的理论和实用价值。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.