Genetic diversity of Plasmodium falciparum and Plasmodium vivax field isolates from the Nowshera district of Pakistan.

IF 2.4 3区医学 Q3 INFECTIOUS DISEASES Malaria Journal Pub Date : 2024-11-25 DOI:10.1186/s12936-024-05190-9

Chandni Hayat, Atif Kamil, Asifullah Khan, Aniqa Sayed, Kehkashan Akbar, Sahib Gul Afridi

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引用次数: 0

Abstract

Background: The genetic diversity of malaria parasites contributes to their ability to adapt to environmental changes, develop drug resistance and circumvent the host immune system. This study aimed to analyse the genetic diversity of the Pfmsp1 and Pfmsp2 genes in Plasmodium falciparum and the Pvmsp-3α gene in Plasmodium vivax isolates from District Nowshera in Pakistan.

Methods: Blood samples from 124 consenting patients with uncomplicated malaria presenting to different hospitals from the Nowshera district were collected between March and August 2019, representing 28 P. falciparum and 96 P. vivax isolates. The genomic DNA extracted from the isolates were subjected to nested PCR and allele-specific analysis. Pvmsp-3α amplified fragments were further treated with restriction fragment length polymorphism (RFLP)-based Hha1 restriction enzyme.

Results: Of the analyzed P. falciparum, 21 distinct alleles were detected, including 14 alleles for Pfmsp-1 and 7 alleles for Pfmsp-2. The sub-allelic families MAD20 (50%) of Pfmsp-1and FC27 (75%) of Pfmsp-2 were predominant. The multiplicity of infection (MOI) was calculated as 1.4 and 1.2 for Pfmsp-1 and Pfmsp-2, respectively, with an overall mean MOI of 1.34. In P. vivax, 4 allelic variants, Pvmsp-3α types A, B, C and D, were detected, while RFLP digestion of amplicons, detected 9 sub-allelic variants (A1-A4, B1, B2, C1, C2 and D1) at the Pvmsp-3α locus.

Conclusion: This first ever report of molecular characterization of P. falciparum and P. vivax genotypes from District Nowshera, Pakistan reveals moderate to high allelic diversity in parasite population from District Nowshera, Pakistan.

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巴基斯坦诺谢拉地区恶性疟原虫和间日疟原虫野外分离物的遗传多样性。

背景：疟原虫的遗传多样性有助于它们适应环境变化、产生抗药性和规避宿主免疫系统的能力。本研究旨在分析恶性疟原虫中 Pfmsp1 和 Pfmsp2 基因的遗传多样性，以及巴基斯坦诺谢拉地区间日疟原虫分离株中 Pvmsp-3α 基因的遗传多样性：方法：收集了2019年3月至8月期间在Nowshera区不同医院就诊的124名无并发症疟疾患者的血液样本，代表28株恶性疟原虫和96株间日疟原虫分离株。从分离物中提取的基因组 DNA 进行了嵌套 PCR 和等位基因特异性分析。用基于限制性片段长度多态性（RFLP）的Hha1限制性酶进一步处理Pvmsp-3α扩增片段：结果：在分析的恶性疟原虫中，检测到 21 个不同的等位基因，包括 14 个 Pfmsp-1 等位基因和 7 个 Pfmsp-2 等位基因。Pfmsp-1的亚等位基因家族MAD20（50%）和Pfmsp-2的亚等位基因家族FC27（75%）占主导地位。根据计算，Pfmsp-1 和 Pfmsp-2 的感染倍率（MOI）分别为 1.4 和 1.2，总平均感染倍率为 1.34。在P. vivax中，检测到4个等位基因变体，即Pvmsp-3α A、B、C和D型，而对扩增子进行RFLP消化后，在Pvmsp-3α基因座上检测到9个亚等位基因变体（A1-A4、B1、B2、C1、C2和D1）：这是对巴基斯坦诺谢拉地区恶性疟原虫和间日疟原虫基因型进行分子鉴定的首次报告，揭示了巴基斯坦诺谢拉地区寄生虫群体中中等至高度的等位基因多样性。

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来源期刊

Malaria Journal 医学-寄生虫学

CiteScore

5.10

自引率

23.30%

发文量

334

审稿时长

2-4 weeks

期刊介绍： Malaria Journal is aimed at the scientific community interested in malaria in its broadest sense. It is the only journal that publishes exclusively articles on malaria and, as such, it aims to bring together knowledge from the different specialities involved in this very broad discipline, from the bench to the bedside and to the field.