Bioactive ECM-mimicking nerve guidance conduit for enhancing peripheral nerve repair

IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Materials Today Bio Pub Date : 2024-11-08 DOI:10.1016/j.mtbio.2024.101324
Shuang Liu , Julia Simińska-Stanny , Lizhao Yan , Lihong Fan , Xiaoyue Ding , Tengda Ma , Wei Guo , Yingsong Zhao , Ming Li , Jianghai Chen , Oseweuba Valentine Okoro , Armin Shavandi , Lei Nie
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Abstract

Extensive research efforts are being directed towards identifying alternatives to autografts for the treatment of peripheral nerve injuries (PNIs) with engineered nerve conduits (NGCs) identified as having potential for PNI patients. These NGCs, however, may not fulfill the necessary criteria for a successful transplant, such as sufficient mechanical structural support and functionalization. To address the aforementioned limitations of NGCs, the present investigation explored the development of double cross-linked hydrogels (o-CSMA-E) that integrate the biocompatibility of porcine tendon extracellular matrix (ECM) with the antimicrobial and conductive properties of methacrylated quaternary chitosan. The hydrogels had matrices that could promote the growth of axons and the transmission of neural signals. The hydrogels were subsequently incorporated into a nanofibrous PLLA-ZnO sheath scaffold (ZnO@PLLA) to emulate the natural nerve structure, guiding cell growth and facilitating nerve regeneration. The collaboration of core and sheath materials in ZnO@PLLA/o-CSMA-E nerve guidance conduits resulted in enhanced migration of Schwann cells, formation of myelin sheaths, and improved locomotion performance in rats with sciatic nerve defects when in vivo studies were undertaken. Notably, the in vivo studies demonstrated the similarity between the newly developed engineered NGCs and autologous transplants, with the newly engineered NGCs possessing the potential to promote functional recovery by mimicking the tubular structure and ECM of nerves.

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生物活性 ECM 仿真神经引导导管,用于加强周围神经修复
目前正在开展广泛的研究工作,以确定治疗周围神经损伤(PNIs)的自体移植替代品,其中工程神经导管(NGCs)被认为具有治疗周围神经损伤患者的潜力。然而,这些 NGC 可能无法满足成功移植的必要条件,如足够的机械结构支持和功能化。为了解决 NGCs 的上述局限性,本研究探讨了双交联水凝胶(o-CSMA-E)的开发,这种水凝胶将猪肌腱细胞外基质(ECM)的生物相容性与甲基丙烯酸化季壳聚糖的抗菌和导电性能融为一体。这些水凝胶具有可促进轴突生长和神经信号传输的基质。这些水凝胶随后被纳入纳米纤维状聚乳酸-氧化锌鞘支架(ZnO@PLLA),以模拟天然神经结构,引导细胞生长并促进神经再生。ZnO@PLLA/o-CSMA-E神经引导导管中的核心材料和鞘材料的合作,增强了许旺细胞的迁移、髓鞘的形成,并在进行体内研究时改善了坐骨神经缺损大鼠的运动能力。值得注意的是,体内研究表明,新开发的工程NGC与自体移植具有相似性,新工程NGC通过模拟神经的管状结构和ECM,具有促进功能恢复的潜力。
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来源期刊
CiteScore
8.30
自引率
4.90%
发文量
303
审稿时长
30 days
期刊介绍: Materials Today Bio is a multidisciplinary journal that specializes in the intersection between biology and materials science, chemistry, physics, engineering, and medicine. It covers various aspects such as the design and assembly of new structures, their interaction with biological systems, functionalization, bioimaging, therapies, and diagnostics in healthcare. The journal aims to showcase the most significant advancements and discoveries in this field. As part of the Materials Today family, Materials Today Bio provides rigorous peer review, quick decision-making, and high visibility for authors. It is indexed in Scopus, PubMed Central, Emerging Sources, Citation Index (ESCI), and Directory of Open Access Journals (DOAJ).
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