The additive effect of 17β-estradiol on the modulation of electrochemotherapy with calcium ions or cisplatin in human clear carcinoma cells

IF 6.9 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL Biomedicine & Pharmacotherapy Pub Date : 2024-11-27 DOI:10.1016/j.biopha.2024.117708

Zofia Łapińska , Nina Rembiałkowska , Anna Szewczyk , Dawid Przystupski , Małgorzata Drąg-Zalesińska , Vitalij Novickij , Jolanta Saczko , Julita Kulbacka , Dagmara Baczyńska

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Abstract

Calcium electroporation (CaEP) is an efficient approach for ovarian cancer treatment. It causes cell death by introducing elevated levels of calcium into cells. In this work, the research focused on two types of cell lines: CHO-K1, representing normal ovary cells, and OvBH-1, representing ovarian clear carcinoma cells. Those cell lines exhibited distinct reactions to calcium electroporation (CaEP). Also, we have evaluated the effects of 17β-estradiol following CaEP and electrochemotherapy (ECT) with cisplatin (CPP). The combination of ECT with CPP and CaEP with prior E₂ preincubation resulted in approximately 23.55 % and 39 % decreases in cell survival compared to the control cells (exposed to CPP and CaCl₂ alone) for ovarian cancer cells. The obtained results showed that ovarian cancer cells preincubated with 17β-estradiol after exposure to pulsed electric fields undergo primary necrosis. Additionally, preincubation of ovarian cancer cells with 17β-estradiol can significantly improve the effectiveness of both chemotherapy and electrochemotherapy involving cisplatin and calcium chloride.

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17β-estradiol 对钙离子或顺铂电化学疗法在人类透明癌细胞中的调节作用的叠加效应

钙电穿孔（CaEP）是治疗卵巢癌的一种有效方法。它通过向细胞中引入高浓度的钙来导致细胞死亡。在这项工作中，研究重点放在两种细胞系上：代表正常卵巢细胞的 CHO-K1 和代表卵巢透明癌细胞的 OvBH-1。这些细胞系对钙电穿孔（CaEP）表现出不同的反应。此外，我们还评估了 17β-estradiol 在 CaEP 和顺铂（CPP）电化学疗法（ECT）之后的作用。与对照细胞（仅暴露于顺铂和 CaCl2）相比，ECT 与 CPP 的结合以及 CaEP 与 E2 预孵育的结合导致卵巢癌细胞的存活率分别下降了约 23.55% 和 39%。结果表明，卵巢癌细胞在暴露于脉冲电场后与 17β-estradiol 预孵育，会发生原发性坏死。此外，用 17β-estradiol 预孵育卵巢癌细胞可显著提高顺铂和氯化钙化疗和电化学疗法的效果。

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来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.