Polyphenolic Antioxidants in Bilberry Stems and Leaves: A Non-Targeted Analysis by Two-Dimensional NMR Spectroscopy and Liquid Chromatography-High-Resolution Mass Spectrometry.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Antioxidants Pub Date : 2024-11-17 DOI:10.3390/antiox13111409
Anna V Faleva, Nikolay V Ulyanovskii, Alexandra A Onuchina, Dmitry S Kosyakov
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Abstract

Compared with those of berries, the stems and leaves of the genus Vaccinium are important and underestimated sources of polyphenols with high antioxidant activity. In the course of this work, aqueous methanol extracts of the aerial parts of common bilberry (Vaccinium myrtillus L.) and bog bilberry (Vaccinium uliginosum L.) were studied to analyze the component compositions of their biologically active polyphenolic compounds. The aqueous methanol fractions of the stems and leaves of the studied samples contained 8.7 and 4.6% extractives, respectively, and were comparable in total polyphenol content, but presented significant differences in antioxidant activity. The identification of polyphenolic compounds was carried out via the following two-stage analytical procedure: (1) non-targeted screening of dominant structures via the 2D NMR method and (2) analysis of HPLC-HRMS data via the scanning of precursor ions for a specific ion. A total of 56 phenolic compounds were identified, including the glycosides quercetin, proanthocyanidins, and catechins, as well as various conjugates of caffeic and p-coumaric acids, including iridoids. Some of the latter, such as caffeoyl and p-coumaroyl hydroxydihydromonotropein, as well as a number of lignan glycosides, were described for the first time in V. uliginósum and V. myrtillus.

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山桑子茎和叶中的多酚抗氧化剂:利用二维核磁共振波谱和液相色谱-高分辨质谱法进行的非靶向分析
与浆果相比,越橘属植物的茎和叶是具有高抗氧化活性的多酚类物质的重要来源,但这种来源被低估了。本研究对普通山桑子(Vaccinium myrtillus L.)和水越橘(Vaccinium uliginosum L.)气生部分的甲醇水提取物进行了研究,以分析其生物活性多酚化合物的成分组成。研究样本的茎和叶的甲醇水溶液馏分分别含有 8.7% 和 4.6% 的萃取物,多酚总含量相当,但抗氧化活性存在显著差异。多酚化合物的鉴定是通过以下两个阶段的分析程序进行的:(1)通过二维核磁共振方法对主要结构进行非靶向筛选;(2)通过扫描特定离子的前体离子对 HPLC-HRMS 数据进行分析。共鉴定出 56 种酚类化合物,包括槲皮素苷、原花青素和儿茶素,以及咖啡酸和对香豆酸的各种共轭物,包括虹彩类。后者中的一些,如咖啡酰基和对香豆酰基羟基二氢香豆素,以及一些木质素苷,是首次在 V. uliginósum 和 V. myrtillus 中发现的。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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