A comparative assesment of biostimulants in microbiome-based ecorestoration of polycyclic aromatic hydrocarbon polluted soil.

Abstract

Polycyclic aromatic hydrocarbons (PAHs) pose severe environmental and public health risks due to their harmful and persistent nature. Therefore, developing sustainable and effective methods for PAH remediation is crucial. This study explores the biostimulation potential of various nutrient supplements in enhancing the metabolic activities of indigenous oleophilic bacteria to PAH degradation and removal. The physicochemical and microbiological characterization of the soil sample obtained from the aged crude oil spill site prior to bioremediation revealed the presence of PAH and other hydrocarbons, reduced nutrient availability as well as an appreciable population of PAH degrading bacteria such as strains of Pseudomonas, Enterobacter, Kosakonia and Staphylococcus. The polluted soil treatment was conducted in six microcosms representing each nutrient supplement: casmes-CM, cocodust-CCD and osmocote-OSM slow-release fertilizers, NPK 20:10:10, casmes + cow dung - CM + CD and a control (unamended soil). Each pot contained 4 kg of soil spiked with 4% Escravos crude oil to a final concentration of 989 mg/kg of PAH, respectively. All treatments enhanced the activity of the indigenous bacteria to promote PAH removal (> 50%) after 35 days although CM + CD had the highest biostimulation effect (B. E.) of 56% with 71.77% PAH attenuation followed by NPK treatment with B. E. of 54.9% and 70.4% PAH removal, respectively. The order of degradation of PAHs from lowest to highest is: control > casmes > osmocote > cocodust > NPK > CM + CD. First-order kinetic model revealed soil microcosm amended with CM + CD had a higher k value (0.0342 day-1) and lower t½ (18.48 day) and this was relatively followed by NPK treated soil. Biostimulation is an effective bioremediation approach to PAH degradation, however, a combined nutrient regimen in the presence of PAH-degrading microbes is more potent and eco-friendly in driving this process.