Continuous chromatography system with 6-zone and 18-column dynamic tandem connection technique for the enrichment of total flavonoids from Epimedium koreanum Nakai.

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS Analytical and Bioanalytical Chemistry Pub Date : 2024-11-28 DOI:10.1007/s00216-024-05669-z
Yi Cui, Jie Huai, Yiting Cai, Shuai Li, Weiye Zhang, Taoyulin Zhou, Yujie Song, Han Zhang, Shengfu Li, Linwu Zhuang, Junjie Zhang
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Abstract

Natural flavonoids have been shown to have many pharmacological activities. Efficient and continuous enrichment of total flavonoids with high content and low mobile phase usage from complex natural products is greatly needed at the moment. In this study, a new continuous chromatography system (CCS) with 6 zones and the 18-column dynamic tandem connection technique was developed and used to enrich total flavonoids from Epimedium koreanum Nakai (EKN). The 18 columns were divided into 6 zones, and the principle of a dynamic series of three columns was adopted for each zone to achieve continuous automatic separation and enrichment of total flavonoids under the control of a logic control valve. The CCS separation conditions were established based on single-column chromatography and a theoretical calculation model of the CCS. By means of the self-designed device and method, 485.11±3.16 g of total flavonoids were isolated from 16.2 kg of EKN. It is worth noting that the total content of 18 types of flavonoids in the samples enriched by the CCS was increased from 2.84±0.07% to 88.29±0.22%, the total recovery rate was 92.20±0.38%, and the RSD of each flavonoid was less than 5.0%. Furthermore, compared with single-column chromatography filled with the same volume of chromatography filler, the entire process saved about 2/3 of the mobile phase usage. In summary, the developed device and method could efficiently and continuously enrich total flavonoids from EKN with high-content and low mobile phase usage and would have a wide application prospect in the separation and enrichment of natural products.

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采用 6 区 18 柱动态串联技术的连续色谱系统用于富集 Epimedium koreanum Nakai 中的总黄酮类化合物。
天然类黄酮已被证明具有多种药理活性。目前亟需从复杂的天然产物中高效、连续地富集高含量、低流动相用量的总黄酮类化合物。本研究开发了一种新型的 6 区连续色谱系统(CCS)和 18 柱动态串联技术,用于富集 Epimedium koreanum Nakai(EKN)中的总黄酮类化合物。18 个色谱柱分为 6 个区,每个区采用三个色谱柱动态串联的原理,在逻辑控制阀的控制下实现总黄酮的连续自动分离和富集。根据单柱色谱法和 CCS 理论计算模型建立了 CCS 分离条件。通过自行设计的装置和方法,从 16.2 公斤 EKN 中分离出 485.11±3.16 克总黄酮。值得注意的是,CCS富集的样品中18种黄酮类化合物的总含量由2.84±0.07%提高到88.29±0.22%,总回收率为92.20±0.38%,每种黄酮类化合物的RSD均小于5.0%。此外,与单柱色谱填充相同体积的色谱填料相比,整个过程节省了约2/3的流动相用量。总之,所开发的装置和方法可以高效、连续地从 EKN 中富集出高含量、低流动相用量的总黄酮类化合物,在天然产物的分离和富集方面具有广阔的应用前景。
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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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