Phytochemical analysis, antioxidant activity and modulatory effect of aqueous leaf extract of Pistacia lentiscus L on oxaliplatin-induced oxidative stress in isolated rat liver mitochondria.

IF 2.1 4区医学 Q3 CHEMISTRY, MULTIDISCIPLINARY Drug and Chemical Toxicology Pub Date : 2024-11-27 DOI:10.1080/01480545.2024.2429615

Nesrine Chouikh, Lamia Benguedouar, Rabha Ayad, Zeineb Medjahed, Andreia Bento-Silva, Noelia Duarte, Mohamed Sifour

{"title":"Phytochemical analysis, antioxidant activity and modulatory effect of aqueous leaf extract of Pistacia lentiscus L on oxaliplatin-induced oxidative stress in isolated rat liver mitochondria.","authors":"Nesrine Chouikh, Lamia Benguedouar, Rabha Ayad, Zeineb Medjahed, Andreia Bento-Silva, Noelia Duarte, Mohamed Sifour","doi":"10.1080/01480545.2024.2429615","DOIUrl":null,"url":null,"abstract":"This study evaluates the antioxidant effects of aqueous leaf extract of Pistacia lentiscus (ALEPL) and its potential to counteract oxaliplatin (OXA)-induced mitochondrial oxidative stress in rat livers, a common side effect of chemotherapy in cancer treatment. Bioactive compounds were identified using High-Performance Liquid Chromatography coupled with Tandem Mass Spectrometry (HPLC-MS and MS), with Fourier-Transform Infrared Spectroscopy (FTIR) and Atomic Absorption Spectrophotometry (AAS) for chemical and mineral analysis. ALEPL showed notable antioxidant activity, with IC50 values of 4.30 ± 0.27 μg/mL for DPPH (2,2-Diphenyl-1-picrylhydrazyl) scavenging, 13.64 ± 0.51 μg/mL for reducing power, 32.62 ± 5.32 μg/mL for hydroxyl radical scavenging, and 205.08 ± 25.77 μg/mL for superoxide anion radical scavenging. In ex vivo experiments, mitochondria isolated from Wistar rat livers were treated with OXA and ALEPL in a dose-dependent manner. ALEPL pretreatment effectively restored mitochondrial antioxidant enzyme activities, increased glutathione (GSH) levels, and reduced lipid peroxidation (MDA) caused by OXA. These findings suggest that ALEPL has the potential to act as a natural antioxidant to support cancer treatment by mitigating chemotherapy-induced oxidative stress. Future studies could explore its application as an adjuvant in clinical settings to enhance the efficacy of chemotherapy while reducing its side effects.","PeriodicalId":11333,"journal":{"name":"Drug and Chemical Toxicology","volume":" ","pages":"1-12"},"PeriodicalIF":2.1000,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Drug and Chemical Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/01480545.2024.2429615","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

This study evaluates the antioxidant effects of aqueous leaf extract of Pistacia lentiscus (ALEPL) and its potential to counteract oxaliplatin (OXA)-induced mitochondrial oxidative stress in rat livers, a common side effect of chemotherapy in cancer treatment. Bioactive compounds were identified using High-Performance Liquid Chromatography coupled with Tandem Mass Spectrometry (HPLC-MS and MS), with Fourier-Transform Infrared Spectroscopy (FTIR) and Atomic Absorption Spectrophotometry (AAS) for chemical and mineral analysis. ALEPL showed notable antioxidant activity, with IC50 values of 4.30 ± 0.27 μg/mL for DPPH (2,2-Diphenyl-1-picrylhydrazyl) scavenging, 13.64 ± 0.51 μg/mL for reducing power, 32.62 ± 5.32 μg/mL for hydroxyl radical scavenging, and 205.08 ± 25.77 μg/mL for superoxide anion radical scavenging. In ex vivo experiments, mitochondria isolated from Wistar rat livers were treated with OXA and ALEPL in a dose-dependent manner. ALEPL pretreatment effectively restored mitochondrial antioxidant enzyme activities, increased glutathione (GSH) levels, and reduced lipid peroxidation (MDA) caused by OXA. These findings suggest that ALEPL has the potential to act as a natural antioxidant to support cancer treatment by mitigating chemotherapy-induced oxidative stress. Future studies could explore its application as an adjuvant in clinical settings to enhance the efficacy of chemotherapy while reducing its side effects.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Pistacia lentiscus L 的植物化学分析、抗氧化活性和叶水提取物对奥沙利铂诱导的离体大鼠肝线粒体氧化应激的调节作用。

本研究评估了Pistacia lentiscus（ALEPL）水性叶提取物的抗氧化作用及其抵消奥沙利铂（OXA）诱导的大鼠肝脏线粒体氧化应激的潜力，氧化应激是癌症治疗中化疗的常见副作用。使用高效液相色谱-串联质谱法（HPLC-MS 和 MS）以及傅立叶变换红外光谱法（FTIR）和原子吸收分光光度法（AAS）进行化学和矿物质分析，对生物活性化合物进行了鉴定。ALEPL 显示出显著的抗氧化活性，其清除 DPPH（2,2-二苯基-1-苦基肼）的 IC50 值为 4.30 ± 0.27 μg/mL，清除还原力的 IC50 值为 13.64 ± 0.51 μg/mL，清除羟自由基的 IC50 值为 32.62 ± 5.32 μg/mL，清除超氧阴离子自由基的 IC50 值为 205.08 ± 25.77 μg/mL。在体外实验中，用 OXA 和 ALEPL 以剂量依赖的方式处理从 Wistar 大鼠肝脏中分离出来的线粒体。ALEPL 预处理可有效恢复线粒体抗氧化酶活性，提高谷胱甘肽（GSH）水平，并降低 OXA 引起的脂质过氧化（MDA）。这些研究结果表明，ALEPL 有可能作为一种天然抗氧化剂，通过减轻化疗引起的氧化应激来支持癌症治疗。未来的研究可以探索将其作为一种辅助药物应用于临床，以提高化疗的疗效，同时减少化疗的副作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Drug and Chemical Toxicology 医学-毒理学

CiteScore

6.00

自引率

3.80%

发文量

审稿时长

3 months

期刊介绍： Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal. Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.