When to Use Rectangular Waveforms in Dielectrophoresis Application to Increase Separation and Sorting Efficiency

IF 2.5 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS ELECTROPHORESIS Pub Date : 2024-11-28 DOI:10.1002/elps.202400164

Niklas P. Boldt, Laura Weirauch, Jana M. Späth, Uwe Kerst, Mario Birkholz, Michael Baune, Roland Thewes

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Abstract

In this study, the influence of using rectangular waveforms is comprehensively investigated on the separation and sorting efficiency of dielectrophoretic (DEP) processes. Besides positive effects on DEP experiments, cases of a diminished force due to rectangular waveforms are investigated and discussed. This investigation encompasses two primary experimental setups. First, microparticle-focusing experiments are carried out using a pair of electrodes within a microfluidic channel. Second, separation experiments are performed using a macroscopic insulator-based dielectrophoretic filter. The study reveals that harmonics of rectangular signals can have a positive impact on separation or sorting efficiency when compared to sinusoidal waveforms, provided that these harmonics contribute to the overall DEP force with the same sign. This positive effect is found to depend on the ratio between the applied fundamental frequency and the cross-over frequency in the Clausius–Mossotti factor. However, violating related derived boundary conditions leads to negative effects and a decrease in the DEP net force.

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何时在介电泳应用中使用矩形波形来提高分离和分拣效率？

本研究全面探讨了使用矩形波形对介质电泳（DEP）过程的分离和分选效率的影响。除了对 DEP 实验的积极影响外，还研究和讨论了矩形波形导致力减弱的情况。这项研究包括两个主要的实验装置。首先，在微流体通道内使用一对电极进行微颗粒聚焦实验。其次，使用基于宏观绝缘体的介电泳滤波器进行分离实验。研究发现，与正弦波相比，矩形信号的谐波会对分离或分拣效率产生积极影响，前提是这些谐波对整个 DEP 力的贡献符号相同。研究发现，这种积极影响取决于应用的基频与克劳修斯-莫索蒂系数中的交叉频率之间的比率。然而，违反相关的导出边界条件则会导致负面效应，并降低 DEP 净力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.