LncRNA SNHG14 Facilitates Cisplatin Resistance Through Upregulating Notch2 via Binding to U2AF2 in Nasopharyngeal Carcinoma.

IF 2.3 3区 医学 Q1 OTORHINOLARYNGOLOGY Head and Neck-Journal for the Sciences and Specialties of the Head and Neck Pub Date : 2024-11-27 DOI:10.1002/hed.28016
Sijia Yan, Puhua Zhang, Shuai Tan, Haiyun Mo, Yanlin Yang
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Abstract

Background: Cisplatin (DDP) is one of the commonly used chemotherapeutic drugs for nasopharyngeal carcinoma (NPC) patients, and the resistance of tumor cells to cisplatin is main obstacle for NPC treatment. This study explored effect and possible mechanism of lncRNA small nucleolar RNA host gene 14 (SNHG14) on drug resistance of NPC cells to cisplatin.

Methods: Levels of SNHG14 and Notch2 in NPC tissues and cells were confirmed using RT-qPCR. Western blot detected Notch2 and ABCB1 expression in NPC cells. IC50 of cisplatin-treated NPC cells was tested utilizing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Cell proliferation and apoptosis were evaluated utilizing colony formation experiment and flow cytometry, respectively. RNA immunoprecipitation (RIP) assay was utilized to validate the target genes of U2AF2. Notch2 mRNA stability was tested using actinomycin D.

Results: SNHG14 level was increased in both cisplatin-resistant NPC tissues and cell lines. SNHG14 silencing in HNE1/DDP cells resulted in inhibition of chemoresistance to cisplatin. Conversely, upregulation of SNHG14 in HNE1 cells enhanced their resistance to cisplatin. SNHG14 exhibited an interaction with U2AF2, leading to stabilization of Notch2 mRNA. Finally, Notch2 was involved in SNHG14-mediated cisplatin resistance in NPC cells.

Conclusion: Our findings demonstrate SNHG14 plays a significant role in promoting chemoresistance of NPC cells to cisplatin through U2AF2/Notch2 axis. These results highlight potential therapeutic targets for NPC treatment.

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LncRNA SNHG14在鼻咽癌中通过与U2AF2结合上调Notch2促进顺铂抗性
背景:顺铂(DDP)是鼻咽癌患者常用的化疗药物之一:顺铂(DDP)是鼻咽癌(NPC)患者常用的化疗药物之一,而肿瘤细胞对顺铂的耐药性是鼻咽癌治疗的主要障碍。本研究探讨了lncRNA小核RNA宿主基因14(SNHG14)对鼻咽癌细胞顺铂耐药性的影响及可能机制:方法:采用RT-qPCR技术确认SNHG14和Notch2在鼻咽癌组织和细胞中的水平。Western印迹检测了NPC细胞中Notch2和ABCB1的表达。利用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)检测顺铂处理的鼻咽癌细胞的 IC50。细胞增殖和凋亡分别通过集落形成实验和流式细胞术进行评估。利用 RNA 免疫沉淀(RIP)试验验证了 U2AF2 的靶基因。使用放线菌素 D 检测 Notch2 mRNA 的稳定性:结果:SNHG14水平在顺铂耐药的鼻咽癌组织和细胞系中均有升高。在 HNE1/DDP 细胞中沉默 SNHG14 可抑制对顺铂的化疗耐药性。相反,HNE1细胞中SNHG14的上调增强了它们对顺铂的耐药性。SNHG14与U2AF2相互作用,导致Notch2 mRNA的稳定。最后,Notch2参与了SNHG14介导的NPC细胞对顺铂的耐药性:我们的研究结果表明,SNHG14在通过U2AF2/Notch2轴促进鼻咽癌细胞对顺铂的化疗耐药性方面发挥着重要作用。这些结果突显了治疗鼻咽癌的潜在靶点。
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来源期刊
CiteScore
7.00
自引率
6.90%
发文量
278
审稿时长
1.6 months
期刊介绍: Head & Neck is an international multidisciplinary publication of original contributions concerning the diagnosis and management of diseases of the head and neck. This area involves the overlapping interests and expertise of several surgical and medical specialties, including general surgery, neurosurgery, otolaryngology, plastic surgery, oral surgery, dermatology, ophthalmology, pathology, radiotherapy, medical oncology, and the corresponding basic sciences.
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