SARS-CoV-2 RNA Presence in Outdoor Air of Public Spaces in Valladolid During Winter, 2021

IF 4.1 2区农林科学 Q2 ENVIRONMENTAL SCIENCES Food and Environmental Virology Pub Date : 2024-11-30 DOI:10.1007/s12560-024-09615-1

Priscilla Gomes da Silva, José Gonçalves, Elisa Rodriguéz, Pedro A. García-Encina, Maria São José Nascimento, Sofia I. V. Sousa, João R. Mesquita

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Abstract

As SARS-CoV-2 continues to evolve and herd immunity establishes, an increasing number of asymptomatic infections have been reported, increasing the risk of airborne spread of the virus. Most of the studies regarding SARS-CoV-2 RNA presence in air refer to indoor environments, with few studies having reported SARS-CoV-2 RNA in outdoor air. The aim of this study was to assess the presence of SARS-CoV-2 RNA at two different settings, crowded outdoor versus empty outdoor environments in Valladolid, Spain, during winter 2021. Using a Coriolis® air sampler, samples were taken from nine different locations within the city center. RNA extraction and a one-step RT-qPCR were carried out. Six out of the 20 air samples were found to be positive, and they were all obtained from crowded outdoor environments. These results highlight that although in less quantity, SARS-CoV-2 RNA is still present in outdoor air, especially at moments of relaxed mitigation efforts and depending on the number of people present.

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2021年冬季巴利亚多利德市公共场所室外空气中SARS-CoV-2 RNA的存在

随着SARS-CoV-2的持续演变和群体免疫的建立，报告的无症状感染越来越多，增加了该病毒通过空气传播的风险。关于空气中SARS-CoV-2 RNA存在的研究大多涉及室内环境，很少有研究报道室外空气中SARS-CoV-2 RNA存在。本研究的目的是评估2021年冬季西班牙巴利亚多利德两种不同环境（拥挤的室外环境和空旷的室外环境）中SARS-CoV-2 RNA的存在。使用科里奥利®空气采样器，从市中心的九个不同地点采集样本。进行RNA提取和一步RT-qPCR。20个空气样本中有6个呈阳性，它们都是在拥挤的室外环境中获得的。这些结果强调，尽管数量减少，但SARS-CoV-2 RNA仍然存在于室外空气中，特别是在缓解努力放松的时候，取决于在场的人数。

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来源期刊

Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.50

自引率

2.90%

发文量

审稿时长

1 months

期刊介绍： Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.