Rita A. S. Dapaah, Laura Ferrer-Font, Xiaoshan Shi, Christopher Hall, Sam Thompson, Larissa Catharina Costa, Peter L. Mage, Aaron J. Tyznik, Kelly Lundsten, Rachael V. Walker
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引用次数: 0
Abstract
Although spectral flow cytometry has become a ubiquitous tool for cell analysis, the use of spectral cytometry on cell sorters requires additional considerations arising from the unique requirements of sorting workflows. Here, we show that care should be taken when ascertaining the purity of a sort on a spectral cell sorter, as the mismatch of buffers used for initial sample suspension and the buffers used for sort collection can affect the unmixing of the data, potentially giving rise to erroneous purity check results.
期刊介绍：
Cytometry Part A, the journal of quantitative single-cell analysis, features original research reports and reviews of innovative scientific studies employing quantitative single-cell measurement, separation, manipulation, and modeling techniques, as well as original articles on mechanisms of molecular and cellular functions obtained by cytometry techniques.
The journal welcomes submissions from multiple research fields that fully embrace the study of the cytome:
Biomedical Instrumentation Engineering
Biophotonics
Bioinformatics
Cell Biology
Computational Biology
Data Science
Immunology
Parasitology
Microbiology
Neuroscience
Cancer
Stem Cells
Tissue Regeneration.
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