Immunohistochemical assessment of ERM proteins (ezrin, radixin, moesin) in the ovaries of different species

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2025-04-01 Epub Date: 2024-11-30 DOI:10.1016/j.tice.2024.102644

Natascha Leitner , Ismi Simsek , Juraj Hlavaty , Sabine Schäfer-Somi , Ingrid Walter

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Abstract

The ezrin/radixin/moesin proteins play a central role in cross-linking plasma membrane proteins with the actin cytoskeleton. Despite intensive ERM protein research in many tissues and pathologies, little is known about these proteins in healthy tissues of reproductive organs. Therefore, we examined ezrin, phosphorylated ezrin/radixin/moesin (pan-pERM), radixin, and moesin distribution at the cellular level by means of immunohistochemistry in ovaries of the following animal species: mouse, dog, cat, sheep, pig, horse, and cynomolgus monkey. Ezrin was expressed in oocytes, ovarian surface, granulosa cells and corpus luteum. A characteristic, predominantly membranous pan-pERM staining pattern was observed in ovarian surface epithelium, oocyte, granulosa cells and corpus luteum. Moesin immunoreactivity was present in all ovarian structures with a prominent signal in endothelial cells of blood vessels. Oocytes, granulosa cells and corpus luteum revealed mainly nuclear radixin staining. Staining pattern and subcellular localization (membranous, cytoplasmic, nuclear) varied between different animal species and between particular ERM proteins as well. This data may help gain new insights into the physiological function of ERM proteins in biological events in the female reproductive system.

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不同物种卵巢ERM蛋白（ezrin, radixin, moesin）的免疫组化评价。

ezrin/radixin/moesin蛋白在质膜蛋白与肌动蛋白细胞骨架交联中起核心作用。尽管在许多组织和病理中对ERM蛋白进行了深入的研究，但对这些蛋白在生殖器官健康组织中的作用知之甚少。因此，我们通过免疫组织化学方法检测了ezrin、磷酸化ezrin/radixin/moesin （pan-pERM）、radixin和moesin在小鼠、狗、猫、羊、猪、马和食蟹猴卵巢中的细胞水平分布。Ezrin在卵母细胞、卵巢表面、颗粒细胞和黄体中均有表达。卵巢表面上皮、卵母细胞、颗粒细胞和黄体呈典型的泛perm染色。Moesin免疫反应性存在于所有卵巢结构中，在血管内皮细胞中有明显的信号。卵母细胞、颗粒细胞和黄体以核放射素染色为主。染色模式和亚细胞定位（膜质、细胞质、细胞核）在不同动物种类和特定ERM蛋白之间也存在差异。这些数据可能有助于对ERM蛋白在女性生殖系统生物事件中的生理功能有新的认识。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.