Hidden target, hidden effects: chlorantraniliprole on the coffee leaf miner (Leucoptera coffeella).

Abstract

Agricultural insecticide usage presents a complex challenge, particularly when addressing hidden targets such as concealed pest species. Typically, insecticide spraying targets either the host plant or the soil substrate, reaching the target when the pests move or feed, yet their vulnerability when concealed remains low. This study delves into the often-obscure effects of insecticides on hidden herbivore species, focusing specifically on the diamide insecticide chlorantraniliprole and its impact on the concealed insect herbivore, the coffee leaf miner Leucoptera coffeella (Guérin-Mèneville & Perrottet) (Lepidoptera: Lyonetidae). We document the progressive effects of chlorantraniliprole from egg-laying through the insect's development and reproductive output. By examining egg-laying preferences, development, survival, respiration rate, leaf consumption, and polysaccharide and protein accumulation, alongside fecundity, in two field-collected leaf miner populations, we elucidate chlorantraniliprole's broader effects. While the insecticide did not alter the leaf miner's egg-laying preferences, one population exhibited higher larval survival, indicating chlorantraniliprole resistance. This chlorantraniliprole-resistant population displayed a lower respiration rate-indicative of reduced stress-and higher leaf consumption, accompanied by increased sugar and protein accumulation. Although this population showed lower adult longevity, it exhibited higher fecundity. These findings highlight the multifaceted impacts of insecticides, extending beyond survival to affect development, fecundity, and potential fitness. Variations in response among insect populations suggest that resistant insects may outperform susceptible ones even under sublethal exposure, with significant implications for management strategies and future outbreaks.