Use of the microalgae Tisochrysis lutea in a "green water" system increases the absorption area in the intestine of larvae of the seahorse Hippocampus reidi Ginsburg, 1933.

IF 2.5 3区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Fish Physiology and Biochemistry Pub Date : 2025-02-01 Epub Date: 2024-12-06 DOI:10.1007/s10695-024-01428-0
Elen Monique de Oliveira Sousa, Nayara Yoshimini de Oliveira, Ianka Agra da Silva, Renata Ávila Ozório, Emilly Monteiro Lopes, Maurício Laterça Martins, Mônica Yumi Tsuzuki
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Abstract

The study aimed to investigate the influence of different microalgae used in green water culture on the intestinal morphology of the seahorse Hippocampus reidi during the first 15 days of life. Four treatments were tested in triplicate, consisting of two microalgae (Tisochrysis lutea-ISO and Chaetoceros muelleri-CHO), used either individually (TISO and TCHO) or combined (TIC 1:1) in larval rearing water, and a treatment without microalgae (TWM). Larvae (6.68 ± 0.55 mm) were fed copepods (Parvocalanus crassirrostris; 2 ind mL-1) and rotifers (Brachionus rotundiformis; 8 ind mL-1) from the first to the seventh day, and from the eighth day onwards, with Artemia sp. nauplii (2 ind mL-1). For the analysis of intestinal histomorphometry, histological analysis of the tissue was performed on day 0 (initial) and after 7 and 15 days. The results demonstrate a significant increase in weight and weight gain of the larvae on the 15th day in the treatments with the addition of the microalgae T. lutea, whether alone or combination with other microalgae, compared to TWM. In relation the intestinal histomorphometry of larvae, the ANCOVA revealed that, on the 7th and 15th day, only the treatments with microalgae significantly affected the surface area of the intestinal villi. The intestinal histomorphometry of larvae from TISO showed higher values of villus height (85.29 ± 1.23 µm2) compared to TWM (69.42 ± 2.82 µm2) and TCHO (76.92 ± 2.23 µm2) (P < 0.05) but did not show significant differences compared to TIC (80.127 ± 2.08 µm2), which reflected on the villi surface area. Therefore, larvae from TISO (3603.43 ± 151.31 µm2) and TIC (3550.29 ± 120.99 µm2) showed significantly higher values of this parameter compared to TWM (3026.79 ± 147.64 µm2) and TCHO (3069.09 ± 126.18 µm2) (P < 0.05). In conclusion, the protocol for H. reidi larvae rearing in a green water system with the addition of the microalgae T. lutea resulted in an increase in the intestinal absorption area, resulting in improved performance of the larvae.

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在“绿水”系统中使用微藻黄体组织溶解增加海马海马幼虫肠道的吸收面积reidi Ginsburg, 1933。
本研究旨在探讨绿水培养中使用的不同微藻对海马海马头15天肠道形态的影响。4个处理分3个重复进行试验,包括两种微藻(黄衣毛藻- iso和穆勒毛藻- cho),分别在幼虫饲养水中使用(TISO和TCHO)或联合使用(TIC 1:1),以及不使用微藻(TWM)。幼虫(6.68±0.55 mm)采食桡足类(Parvocalanus crassirostris;2和mL-1)和轮虫(轮状臂轮虫;从第1天到第7天,从第8天开始,用Artemia sp. nauplii(2和mL-1)。为进行肠组织形态学分析,分别在第0天(初始)、第7天和第15天对组织进行组织学分析。结果表明,与TWM相比,单独添加或与其他微藻组合处理的黄叶微藻在第15天的幼虫体重和增重均显著增加。在第7天和第15天,ANCOVA结果显示,只有微藻处理对仔鱼肠绒毛表面积有显著影响。通过肠道组织形态学测定,TISO组幼虫的绒毛高度(85.29±1.23µm2)高于TWM组(69.42±2.82µm2)和TCHO组(76.92±2.23µm2) (P < 2),这反映在绒毛表面积上。因此,TISO(3603.43±151.31µm2)和TIC(3550.29±120.99µm2)幼虫的该参数值显著高于TWM(3026.79±147.64µm2)和TCHO(3069.09±126.18µm2) (P < 0.05)
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来源期刊
Fish Physiology and Biochemistry
Fish Physiology and Biochemistry 农林科学-生化与分子生物学
CiteScore
5.60
自引率
6.90%
发文量
106
审稿时长
4 months
期刊介绍: Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.
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