Determination of blood biochemical indices and research of egg quality-related candidate gene CDH5 in Putian black duck.

IF 2.6 3区生物学 Q2 GENETICS & HEREDITY Gene Pub Date : 2025-02-10 Epub Date: 2024-12-04 DOI:10.1016/j.gene.2024.149142

Huihuang Li, Yue Yang, Fan Yang, Xinguo Bao, Chengfu Pan, Weilong Lin, Lianjie Lai, Weimin Lin, Ruiyi Lin

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Abstract

Improving egg quality and enhancing production efficiency are essential goals in poultry breeding. CDH5 encodes a cadherin involved in Ca²⁺ transport in endothelial cells. The role of CDH5 in regulating duck egg quality and its mechanisms affecting Ca²⁺ concentrations in duck uterine epithelial cells remains unclear. This study evaluated egg quality traits of the Putian black duck and conducted an association analysis with blood biochemical indices and single nucleotide polymorphisms in CDH5. Additionally, we constructed a CDH5 overexpression vector and synthesized specific siRNAs for transfection into Putian black duck uterine epithelial cells to assess Ca²⁺ concentrations. Our results revealed a significant association between egg quality and five novel SNPs in CDH5, along with various blood biochemical indices. Further experiments demonstrated that CDH5 overexpression and knockdown reduced Ca²⁺ concentrations in the uterine epithelial cells of Putian black ducks.

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莆田黑鸭血液生化指标测定及蛋品质相关候选基因CDH5的研究。

提高蛋品质量和生产效率是家禽育种的重要目标。CDH5编码一种在内皮细胞中参与Ca2+运输的钙粘蛋白。CDH5在鸭蛋品质调控中的作用及其影响鸭子宫上皮细胞Ca2+浓度的机制尚不清楚。本研究评价了莆田黑鸭的蛋品质性状，并对其与血液生化指标和CDH5基因单核苷酸多态性进行了相关性分析。此外，我们构建了CDH5过表达载体，并合成了特异性sirna，转染莆田黑鸭子宫上皮细胞，以评估Ca2+浓度。我们的研究结果显示，卵子质量与CDH5的五个新snp以及各种血液生化指标之间存在显著关联。进一步实验表明，CDH5过表达和敲低可降低莆田黑鸭子宫上皮细胞Ca2+浓度。

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来源期刊

Gene 生物-遗传学

CiteScore

6.10

自引率

2.90%

发文量

718

审稿时长

42 days

期刊介绍： Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.