Determination of blood biochemical indices and research of egg quality-related candidate gene CDH5 in Putian black duck.

IF 2.6 3区生物学 Q2 GENETICS & HEREDITY Gene Pub Date : 2025-02-10 Epub Date: 2024-12-04 DOI:10.1016/j.gene.2024.149142

Huihuang Li, Yue Yang, Fan Yang, Xinguo Bao, Chengfu Pan, Weilong Lin, Lianjie Lai, Weimin Lin, Ruiyi Lin

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引用次数: 0

Abstract

Improving egg quality and enhancing production efficiency are essential goals in poultry breeding. CDH5 encodes a cadherin involved in Ca²⁺ transport in endothelial cells. The role of CDH5 in regulating duck egg quality and its mechanisms affecting Ca²⁺ concentrations in duck uterine epithelial cells remains unclear. This study evaluated egg quality traits of the Putian black duck and conducted an association analysis with blood biochemical indices and single nucleotide polymorphisms in CDH5. Additionally, we constructed a CDH5 overexpression vector and synthesized specific siRNAs for transfection into Putian black duck uterine epithelial cells to assess Ca²⁺ concentrations. Our results revealed a significant association between egg quality and five novel SNPs in CDH5, along with various blood biochemical indices. Further experiments demonstrated that CDH5 overexpression and knockdown reduced Ca²⁺ concentrations in the uterine epithelial cells of Putian black ducks.

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来源期刊

Gene 生物-遗传学

CiteScore

6.10

自引率

2.90%

发文量

718

审稿时长

42 days

期刊介绍： Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.