Lipidomic profiling of the febrile rat hypothalamus by the intervention of Artemisia japonica extracts.

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL Journal of pharmaceutical and biomedical analysis Pub Date : 2025-03-15 Epub Date: 2024-11-28 DOI:10.1016/j.jpba.2024.116588

Xiaodong Zhang, Suqing Zhao, Yuxue Ma, Wen Kang, Wenbin Zhou, Chen Zhang, Zeper Abliz

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Abstract

Artemisia species have been regarded as an important source of ethnic medicinal plants, such as A. annua and A. capillaris, both of which are widely used in clinical treatment. The clinical efficacy of A. japonica is similar to that of A. capillaris, but fewer pharmaceutical studies have been reported. Given that the extracts of A. japonica were observed to reduce the rectal temperature of febrile rats induced by LPS, this study was designed to demonstrate this regulatory effect of the extracts, with a particular focus on the lipidomic profiling of the febrile rat hypothalamus. A total of 72 differential metabolites were filtered out and the association between lipid profiling and potential mechanism was explored. Sphingolipid, glycerophospholipid, arachidonic acid and ether lipid metabolism pathways were significantly enriched. TNF-α, IL-6 and PGE₂ cytokines in the hypothalamus were significantly downregulated by the intervention of the extracts of A. japonica. Enzymatic reaction enrichment analysis suggested that PEMT and COX-2 might be potential targets of the efficacy, and which were testified to be downregulated by the ELISA assay under the extracts intervention.

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青蒿提取物对发热大鼠下丘脑脂质组学的影响。

青蒿属植物被认为是民族药用植物的重要来源，如A. annua和A. capillaris，均被广泛应用于临床治疗。其临床疗效与毛缕草相似，但药物研究报道较少。考虑到粳米提取物可以降低LPS诱导的发热大鼠的直肠温度，本研究旨在证明粳米提取物的这种调节作用，并特别关注发热大鼠下丘脑的脂质组学特征。共筛选出72种差异代谢物，并探讨脂质分析与潜在机制之间的关系。鞘脂、甘油磷脂、花生四烯酸和醚类脂质代谢途径显著富集。下丘脑TNF-α、IL-6、PGE2等细胞因子在粳米提取物干预下显著下调。酶促反应富集分析提示ppt和COX-2可能是药效的潜在靶点，ELISA检测证实在提取物干预下ppt和COX-2下调。

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.