Rastislav Boško, Helena Pluháčková, Jan Martiník, Karolína Benešová, Zdeněk Svoboda, Sylvie Běláková, Marek Pernica
{"title":"Occurrence of mycotoxins in milk thistle: to be included in legislation or not?","authors":"Rastislav Boško, Helena Pluháčková, Jan Martiník, Karolína Benešová, Zdeněk Svoboda, Sylvie Běláková, Marek Pernica","doi":"10.1007/s12550-024-00577-8","DOIUrl":null,"url":null,"abstract":"<p><p>The silymarin complex extracted from milk thistle provides significant health benefits, particularly due to its antioxidant and hepatoprotective properties. However, plant substances can be contaminated by a number of fungi types and their secondary metabolites-mycotoxins. This work deals with the determination of aflatoxins and zearalenone and its metabolites in 39 different samples grown in 2020 and 2021. Analysis of mycotoxins was performed by UHPLC-MS/MS after immunoaffinity column AFLAPREP<sup>®</sup> and EASI-EXTRACT<sup>®</sup> ZEARALENONE clean-up. The presence of aflatoxins was not confirmed in the monitored samples, but 1/3 of the samples were contaminated with zearalenone in the range of 2.8-378.9 µg/kg. Metabolites of zearalenone such as α-zearalenol, α-zearalanol, and β-zearalanol were not detected in any of the samples. β-Zearalenol was found in two samples (2.6 µg/kg and 29.8 µg/kg).</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycotoxin Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12550-024-00577-8","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The silymarin complex extracted from milk thistle provides significant health benefits, particularly due to its antioxidant and hepatoprotective properties. However, plant substances can be contaminated by a number of fungi types and their secondary metabolites-mycotoxins. This work deals with the determination of aflatoxins and zearalenone and its metabolites in 39 different samples grown in 2020 and 2021. Analysis of mycotoxins was performed by UHPLC-MS/MS after immunoaffinity column AFLAPREP® and EASI-EXTRACT® ZEARALENONE clean-up. The presence of aflatoxins was not confirmed in the monitored samples, but 1/3 of the samples were contaminated with zearalenone in the range of 2.8-378.9 µg/kg. Metabolites of zearalenone such as α-zearalenol, α-zearalanol, and β-zearalanol were not detected in any of the samples. β-Zearalenol was found in two samples (2.6 µg/kg and 29.8 µg/kg).
期刊介绍:
Mycotoxin Research, the official publication of the Society for Mycotoxin Research, is a peer-reviewed, scientific journal dealing with all aspects related to toxic fungal metabolites. The journal publishes original research articles and reviews in all areas dealing with mycotoxins. As an interdisciplinary platform, Mycotoxin Research welcomes submission of scientific contributions in the following research fields:
- Ecology and genetics of mycotoxin formation
- Mode of action of mycotoxins, metabolism and toxicology
- Agricultural production and mycotoxins
- Human and animal health aspects, including exposure studies and risk assessment
- Food and feed safety, including occurrence, prevention, regulatory aspects, and control of mycotoxins
- Environmental safety and technology-related aspects of mycotoxins
- Chemistry, synthesis and analysis.