Occurrence of mycotoxins in milk thistle: to be included in legislation or not?

IF 3.1 4区 医学 Q2 MYCOLOGY Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-12-10 DOI:10.1007/s12550-024-00577-8
Rastislav Boško, Helena Pluháčková, Jan Martiník, Karolína Benešová, Zdeněk Svoboda, Sylvie Běláková, Marek Pernica
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Abstract

The silymarin complex extracted from milk thistle provides significant health benefits, particularly due to its antioxidant and hepatoprotective properties. However, plant substances can be contaminated by a number of fungi types and their secondary metabolites-mycotoxins. This work deals with the determination of aflatoxins and zearalenone and its metabolites in 39 different samples grown in 2020 and 2021. Analysis of mycotoxins was performed by UHPLC-MS/MS after immunoaffinity column AFLAPREP® and EASI-EXTRACT® ZEARALENONE clean-up. The presence of aflatoxins was not confirmed in the monitored samples, but 1/3 of the samples were contaminated with zearalenone in the range of 2.8-378.9 µg/kg. Metabolites of zearalenone such as α-zearalenol, α-zearalanol, and β-zearalanol were not detected in any of the samples. β-Zearalenol was found in two samples (2.6 µg/kg and 29.8 µg/kg).

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水飞蓟中真菌毒素的发生:应不应纳入立法?
从水飞蓟中提取的水飞蓟素复合物具有显著的健康益处,特别是由于其抗氧化和保护肝脏的特性。然而,植物物质可能被许多真菌类型及其次生代谢物真菌毒素污染。这项工作涉及在2020年和2021年生长的39个不同样品中黄曲霉毒素和玉米赤霉烯酮及其代谢物的测定。免疫亲和柱AFLAPREP®和EASI-EXTRACT®ZEARALENONE清洗后,采用UHPLC-MS/MS进行真菌毒素分析。监测样品中未发现黄曲霉毒素,但有1/3的样品被玉米赤霉烯酮污染,污染范围为2.8 ~ 378.9µg/kg。玉米赤霉烯酮的代谢物如α-玉米赤霉烯醇、α-玉米赤霉烯醇和β-玉米赤霉烯醇均未在任何样品中检测到。在两个样品中分别发现了β-玉米赤霉烯醇(2.6µg/kg和29.8µg/kg)。
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来源期刊
Mycotoxin Research
Mycotoxin Research MYCOLOGYTOXICOLOGY-TOXICOLOGY
CiteScore
6.40
自引率
6.70%
发文量
29
期刊介绍: Mycotoxin Research, the official publication of the Society for Mycotoxin Research, is a peer-reviewed, scientific journal dealing with all aspects related to toxic fungal metabolites. The journal publishes original research articles and reviews in all areas dealing with mycotoxins. As an interdisciplinary platform, Mycotoxin Research welcomes submission of scientific contributions in the following research fields: - Ecology and genetics of mycotoxin formation - Mode of action of mycotoxins, metabolism and toxicology - Agricultural production and mycotoxins - Human and animal health aspects, including exposure studies and risk assessment - Food and feed safety, including occurrence, prevention, regulatory aspects, and control of mycotoxins - Environmental safety and technology-related aspects of mycotoxins - Chemistry, synthesis and analysis.
期刊最新文献
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