Optimized production of laccase from Pseudomonas stutzeri and its biodegradation of lignin in biomass.

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Folia microbiologica Pub Date : 2024-12-11 DOI:10.1007/s12223-024-01232-6
Waqar Rasool Minhas, Saira Bashir, Cheng Zhang, Ahmad Raza
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引用次数: 0

Abstract

Laccases are multi-copper oxidases that play an important role in the biodegradation of phenolic compounds, lignin, dye, and wastes. Here, we report the screening of potential laccase-producing indigenous bacterial isolates and subsequent optimization of laccase production using crop residues as cheap supplementary energy sources. Among 16 bacterial isolates, seven were selected based on the appearance of reddish-brown bacterial colonies and guaiacol oxidation assay after 10 days of incubation at 37 °C. The maximum laccase activity (2.755 U/mL) was observed for bacterial isolate WR2. Response surface methodology (RSM) was used to maximize laccase production from WR2, identified as Pseudomonas stutzeri. Plackett-Burman design (PBD) was employed to design production runs involving various factors including time, pH, inoculum, wheat straw, cotton stalk, wheat bran, rice straw, copper sulfate, sugarcane bagasse, yeast extract, and peptone. The interactions of different factors were analyzed from the responses (laccase enzyme activity, etc.) in 12 experimental runs. In experimental run 4, the maximum laccase enzymatic activity (1.86 U/mL) was achieved after a 10-day incubation with wheat straw (1%) and cotton stalk (1%) at pH 6.8 and 37 °C, and high-degree lignin degradation was evident from a substantial reduction in the FTIR aromatic stretching peak of the degraded biomass.

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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
期刊最新文献
Anticancer effect of a combinatorial treatment of 5-fluorouracil and cell extract of some probiotic lactobacilli strains isolated from camel milk on colorectal cancer cells. Characterization of Archaea membrane lipids in radioactive springs using shotgun lipidomics. Prolonged fever in a 13-year-old girl misdiagnosed as Ochrobactrum spp. bacteremia-the pitfalls of diagnostic Brucella spondylitis in a non-endemic country. Optimized production of laccase from Pseudomonas stutzeri and its biodegradation of lignin in biomass. Correction to: Characteristics and in vitro properties of potential probiotic strain Fructobacillus tropaeoli KKP 3032 isolated from orange juice.
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