Isolation, characterization and biological activities of phenolics from the roots bark of Morus alba

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Phytochemistry Pub Date : 2024-12-09 DOI:10.1016/j.phytochem.2024.114352

Min Tan , Xiao-Cong Liu , Dan Liu, Xuan-Qin Chen, Rong-Tao Li, Zhi-Jun Zhang

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Abstract

An undescribed coumarin–limonene hybrid (morusalin A, 1), an unreported steppogenin–limonene hybrid (morusalin B, 2), and three undescribed isoprenylated flavonoids (morusalins C–E, 3–5), along with forty-one known ones (6–46), were isolated from the dried roots bark of Morus alba. Their structures with absolute configurations were elucidated by detailed interpretation of NMR spectroscopy, mass spectrometry, single-crystal X-ray diffraction, and ECD calculations. In addition, all these isolated compounds were assayed for their inhibitory activity against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in murine macro-phage RAW 264.7 cells and for their cytotoxic activities against human colon cancer (HCT 8) and hepatoma (HepG 2) cells. Moreover, a biosynthetic pathway for the formation of compounds 1–6 is also proposed.

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桑树根皮中酚类物质的分离、表征及生物活性研究。

从桑树的干根树皮中分离出一种未被描述的香豆素-柠檬烯杂交体（morusalin A, 1），一种未被报道的steppogenin-柠檬烯杂交体（morusalin B, 2）和三种未被描述的异戊烯化黄酮类化合物（morusalins C-E, 3-5），以及41种已知的黄酮类化合物（6-46）。通过核磁共振谱、质谱、单晶x射线衍射和ECD计算，对它们的绝对构型结构进行了详细的解释。此外，我们还检测了所有分离得到的化合物对脂多糖（LPS）诱导的小鼠巨噬细胞RAW 264.7细胞一氧化氮（NO）产生的抑制活性以及对人结肠癌（HCT 8）和肝癌（HepG 2）细胞的细胞毒活性。此外，还提出了化合物1-6形成的生物合成途径。

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.

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