Properties of Compounds Citral and Geraniol on Trichomonas gallinae: Activity in Vitro and Cytotoxicity.

IF 0.9 4区医学 Q4 PARASITOLOGY Iranian Journal of Parasitology Pub Date : 2024-10-01 DOI:10.18502/ijpa.v19i4.17164

Bruna Baccega, Juliana Montelli Fenalti, Alexia Brauner de Mello, Yan Wahast Islabão, Paloma T Birmann, Alessandra Neis, Marjorie de Giacometi, Francielle Liz Monteiro, Silvia de Oliveira Hubner, Rodrigo de Almeida Vaucher, Nara Amélia da Rosa Farias, Roghayeh Norouzi, Abolghasem Siyadatpanah, Camila Belmonte Oliveira

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引用次数: 0

Abstract

Background: Avian trichomoniasis is an important disease that causes bird mortality, both wild and captive, around the world. This study evaluated the in vitro cytotoxicity, antioxidant, and antiparasitic activity of citral (3.7-Dimetil-2.6-octadienal) and geraniol (trans-3.7-Dimetil-2.6-octadien-1-ol) against Trichomonas gallinae trophozoites.

Methods: In vitro assays were conducted at the Laboratory of Protozoology and Entomology (LAPEN) at the Federal University of Pelotas (UFPel), Brazil in 2019 using tests with 10⁶ parasites and citral and geraniol at concentrations ranging from 10 to 100 μM and four controls: NC (culture medium and trophozoites), MTZ (trophozoites plus 100 μM of metronidazole), and TW (trophozoites plus vehicles used for solubilizing derivatives (0.01% Tween).

Results: The citral (60 μM) and geraniol (50 μM) concentrations reduced the trophozoites's viability by 100%. The molecular docking experiment demonstrated that citral and geraniol might inhibit a hydrogen enzyme for T. gallinae survival.

Conclusion: The major compounds of lemongrass have potential antitrichomonal activity against T. gallinae in vitro.

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柠檬醛和香叶醇对鸡毛滴虫的体外活性及细胞毒性研究。

背景：禽滴虫病是世界范围内引起野生和圈养鸟类死亡的一种重要疾病。本研究评价了柠檬醛（3.7-二甲醚-2.6-八烯二醛）和香叶醇（反式3.7-二甲醚-2.6-八烯二烯-1-醇）对鸡毛滴虫滋养体的体外细胞毒性、抗氧化和抗寄生活性。方法：2019年在巴西佩洛塔斯联邦大学（UFPel）原生动物与昆虫学实验室（LAPEN）进行体外实验，采用106种寄生虫和浓度为10 ~ 100 μM的柠檬醛和香叶醇，以及4种对照：NC（培养基和滋养体）、MTZ（滋养体加100 μM甲硝唑）和TW（滋养体加增溶衍生物载体（0.01% Tween））。结果：柠檬醛（60 μM）和香叶醇（50 μM）浓度使滋养体活力降低100%。分子对接实验表明，柠檬醛和香叶醇可能抑制一种氢酶，使鸡瘿菌存活。结论：柠檬草中主要化合物对鸡滴虫具有潜在的体外抗滴虫活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Parasitology PARASITOLOGY-

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.