Comprehensive Quality Evaluation of Traditional Chinese Medicine Guanxinjing Capsule Based on Fingerprint and Multicomponent Simultaneous Quantitative Method

IF 2.8 3区工程技术 Q2 CHEMISTRY, ANALYTICAL Journal of separation science Pub Date : 2025-01-08 DOI:10.1002/jssc.70059

Guanhua Zhang, Xin Liu, Hao Liang, Xu Wang, Qi Wang, Qiao Wang

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Abstract

A novel dual-wavelength ultrahigh performance liquid chromatography (UHPLC) fingerprint was established, 56 common peaks were confirmed and attributed to the source of the medicinal materials, and 13 chromatographic peaks of them were identified by UHPLC quadrupole time-of-flight (Q-TOF)-MS/MS and UHPLC-UV method. Furthermore, a simple and sensitive HPLC-quadrupole trap (Q-TRAP)-MS/MS was developed for the simultaneous determination of 16 active components with electrospray ionization (ESI) source switching between positive and negative modes in a single run. The above two methods were successfully applied for the quality evaluation of Guanxinjing capsule (GXJC). Finally, statistical analysis including principal component analysis (PCA), hierarchical cluster analysis (HCA), and orthogonal partial least squares discriminate analysis (OPLS-DA) were applied, and 13 variables with great contribution to different groups of samples were screened. This study not only provides a rapid, accurate, and comprehensive qualitative and quantitative method for quality evaluation of GXJC but it also provides a reference workflow for the other traditional Chinese medicines.

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基于指纹图谱和多组分同时定量法的中药冠心静胶囊质量综合评价

建立了一种新型的双波长超高效液相色谱（UHPLC）指纹图谱，确认了56个共同峰，并归属于药材的来源，其中13个色谱峰通过UHPLC四极杆飞行时间(Q-TOF)-MS/MS和UHPLC- uv法进行了鉴定。在此基础上，建立了一种简便、灵敏的高效液相色谱-四极阱(Q-TRAP)-质谱联用技术，在电喷雾电离（ESI）源的正负切换模式下，在一次运行中同时测定16种有效成分。上述两种方法均可用于冠心静胶囊的质量评价。最后，采用主成分分析（PCA）、层次聚类分析（HCA）和正交偏最小二乘判别分析（OPLS-DA）进行统计分析，筛选出13个对不同样本组贡献较大的变量。本研究不仅为GXJC的质量评价提供了快速、准确、全面的定性和定量方法，也为其他中药的质量评价提供了参考工作流程。

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来源期刊

Journal of separation science 化学-分析化学

CiteScore

6.30

自引率

16.10%

发文量

408

审稿时长

1.8 months

期刊介绍： The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.