Protocol for microinjection of rapamycin into the zebrafish habenula.

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS STAR Protocols Pub Date : 2025-01-11 DOI:10.1016/j.xpro.2024.103566
Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska
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引用次数: 0

Abstract

Mechanistic target of rapamycin complex 1 (mTorC1) activity plays a crucial role in brain development. Here, we present an approach for rapamycin microinjection into the habenula of larval zebrafish to achieve localized inhibition of the mTorC1 pathway and explore the role of mTorC1 in habenula function. We describe steps for performing microinjections and maintaining zebrafish larvae before and after the procedure. For complete details on the use and execution of this protocol, please refer to Doszyn et al.1.

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斑马鱼缰内微量注射雷帕霉素的方法。
雷帕霉素复合体1 (mTorC1)活性的机制靶点在大脑发育中起着至关重要的作用。在此,我们提出了一种将雷帕霉素显微注射到斑马鱼幼鱼的habenula中,以实现对mTorC1通路的局部抑制,并探索mTorC1在habenula功能中的作用。我们描述了在手术前后进行显微注射和维持斑马鱼幼虫的步骤。有关本协议使用和执行的完整细节,请参见Doszyn等人1。
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来源期刊
STAR Protocols
STAR Protocols Biochemistry, Genetics and Molecular Biology-General Biochemistry, Genetics and Molecular Biology
CiteScore
2.00
自引率
0.00%
发文量
789
审稿时长
10 weeks
期刊介绍:
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