Genomic analysis and replication kinetics of the closely related EHV-1 neuropathogenic 21P40 and abortigenic 97P70 strains.

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES Veterinary Research Pub Date : 2025-01-13 DOI:10.1186/s13567-024-01434-3
Eslam Mohamed, Ines Zarak, Nick Vereecke, Sebastiaan Theuns, Kathlyn Laval, Hans Nauwynck
{"title":"Genomic analysis and replication kinetics of the closely related EHV-1 neuropathogenic 21P40 and abortigenic 97P70 strains.","authors":"Eslam Mohamed, Ines Zarak, Nick Vereecke, Sebastiaan Theuns, Kathlyn Laval, Hans Nauwynck","doi":"10.1186/s13567-024-01434-3","DOIUrl":null,"url":null,"abstract":"<p><p>Varicellovirus equidalpha 1, formerly known as Equid alphaherpesvirus 1 (EHV-1), is highly prevalent and can lead to various problems, such as respiratory problems, abortion, neonatal foal death, and neurological disorders. The latter is known as equine herpes myeloencephalopathy (EHM). Cases of EHM have significantly increased since the beginning of the twenty-first century. The genomic sequences of five isolates associated with the fatal neurological outbreak in Valencia, Spain, in 2021 were analyzed and documented. The genome and replication kinetics of the Belgian EHM isolate 21P40, associated with the Valencia outbreak, and the well-characterized abortigenic strain 97P70 were compared. Both strains exhibited a nucleotide identity of 99.96%, with only seven genetic mutations in ORFs 13, 24, 30, 32, 40, 65, and 71. Isoleucine and asparagine at loci 291 and 207 of ORF30 (DNA polymerase) and ORF65 (ICP22), respectively, were unique to isolates from the Valencia outbreak. The replication kinetics of these two genetically closely related strains were determined in rabbit kidney (RK-13), equine respiratory, and vaginal mucosal explant cells, as well as equine blood monocytes (CD172a<sup>+</sup>). Both strains replicated equally well in RK-13 cells. The neuropathogenic isolate 21P40 exhibited a more extensive infection in respiratory explants and blood monocytes, as demonstrated by more plaques and single infected leukocytes, and a higher percentage of infected monocytes. In contrast, vaginal explants infected with the abortigenic strain 97P70 demonstrated more plaques and single infected leukocytes. In conclusion, 21P40 replicated significantly different compared to 97P70 but shared similarities with the Belgian well-studied neuropathogenic EHV-1 strain 03P37.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"12"},"PeriodicalIF":3.7000,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731145/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s13567-024-01434-3","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Varicellovirus equidalpha 1, formerly known as Equid alphaherpesvirus 1 (EHV-1), is highly prevalent and can lead to various problems, such as respiratory problems, abortion, neonatal foal death, and neurological disorders. The latter is known as equine herpes myeloencephalopathy (EHM). Cases of EHM have significantly increased since the beginning of the twenty-first century. The genomic sequences of five isolates associated with the fatal neurological outbreak in Valencia, Spain, in 2021 were analyzed and documented. The genome and replication kinetics of the Belgian EHM isolate 21P40, associated with the Valencia outbreak, and the well-characterized abortigenic strain 97P70 were compared. Both strains exhibited a nucleotide identity of 99.96%, with only seven genetic mutations in ORFs 13, 24, 30, 32, 40, 65, and 71. Isoleucine and asparagine at loci 291 and 207 of ORF30 (DNA polymerase) and ORF65 (ICP22), respectively, were unique to isolates from the Valencia outbreak. The replication kinetics of these two genetically closely related strains were determined in rabbit kidney (RK-13), equine respiratory, and vaginal mucosal explant cells, as well as equine blood monocytes (CD172a+). Both strains replicated equally well in RK-13 cells. The neuropathogenic isolate 21P40 exhibited a more extensive infection in respiratory explants and blood monocytes, as demonstrated by more plaques and single infected leukocytes, and a higher percentage of infected monocytes. In contrast, vaginal explants infected with the abortigenic strain 97P70 demonstrated more plaques and single infected leukocytes. In conclusion, 21P40 replicated significantly different compared to 97P70 but shared similarities with the Belgian well-studied neuropathogenic EHV-1 strain 03P37.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
密切相关的EHV-1神经致病性21P40和流产性97P70菌株的基因组分析和复制动力学。
马α 1型水痘病毒,以前称为1型马α疱疹病毒(EHV-1),非常流行,可导致各种问题,如呼吸系统问题、流产、新生马驹死亡和神经系统疾病。后者被称为马疱疹髓脑病(EHM)。自21世纪初以来,EHM病例显著增加。分析并记录了与2021年西班牙瓦伦西亚致命神经系统疫情相关的5个分离株的基因组序列。我们比较了与瓦伦西亚爆发相关的比利时EHM分离株21P40和具有良好特征的流产菌株97P70的基因组和复制动力学。两株菌株的核苷酸同源性为99.96%,仅在orf 13、24、30、32、40、65和71中发生了7个基因突变。ORF30 (DNA聚合酶)和ORF65 (ICP22)基因座291和207处的异亮氨酸和天冬酰胺分别为瓦伦西亚暴发的分离株所特有。在兔肾(RK-13)、马呼吸道和阴道粘膜外植体细胞以及马血液单核细胞(CD172a+)中测定了这两种遗传密切相关的菌株的复制动力学。这两种菌株在RK-13细胞中复制得同样好。神经致病性分离物21P40在呼吸道外植体和血液单核细胞中表现出更广泛的感染,表现为更多的斑块和单个感染的白细胞,以及更高比例的感染单核细胞。相比之下,流产菌株97P70感染的阴道外植体显示出更多的斑块和单个感染的白细胞。综上所述,与97P70相比,21P40的复制性有显著差异,但与比利时研究充分的神经致病性EHV-1菌株03P37有相似之处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
期刊最新文献
GHSR gene knockout alleviates the liver pathological response in Echinococcus granulosus infection by reducing parasite survival. Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China. Transcriptomic analysis revealed ferroptosis in ducklings with splenic necrosis induced by NDRV infection. The striking incidence of animal listeriosis in Germany (2014-2024) indicates a persistent but neglected risk for One Health. Characterization of a recombinant Sendai virus vector encoding the small ruminant lentivirus gag-P25: antiviral properties in vitro and transgene expression in sheep.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1