{"title":"Continuous selection in E. coli to identify cyclic peptide inhibitors of protein aggregation","authors":"","doi":"10.1038/s41589-024-01824-w","DOIUrl":null,"url":null,"abstract":"Targeting of protein aggregates is technologically challenging. We developed a phage-assisted continuous evolution platform for rapid selection of protein aggregation inhibitors from genetically encoded cyclic peptide libraries in Escherichia coli. This strategy enabled discovery of cyclic peptides that suppress the aggregation of two clinically relevant proteins, amyloid-β42 (Aβ42) and human islet amyloid polypeptide (hIAPP).","PeriodicalId":18832,"journal":{"name":"Nature chemical biology","volume":"37 1","pages":""},"PeriodicalIF":12.9000,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature chemical biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1038/s41589-024-01824-w","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Targeting of protein aggregates is technologically challenging. We developed a phage-assisted continuous evolution platform for rapid selection of protein aggregation inhibitors from genetically encoded cyclic peptide libraries in Escherichia coli. This strategy enabled discovery of cyclic peptides that suppress the aggregation of two clinically relevant proteins, amyloid-β42 (Aβ42) and human islet amyloid polypeptide (hIAPP).
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Nature Chemical Biology stands as an esteemed international monthly journal, offering a prominent platform for the chemical biology community to showcase top-tier original research and commentary. Operating at the crossroads of chemistry, biology, and related disciplines, chemical biology utilizes scientific ideas and approaches to comprehend and manipulate biological systems with molecular precision.
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