Effect of nutritional stress and serum starvation on the optical absorbance of normal and malignant epithelial cell lines.

IF 2.4 4区医学 Q3 ENGINEERING, BIOMEDICAL Lasers in Medical Science Pub Date : 2025-01-23 DOI:10.1007/s10103-024-04254-1

Juliana Stephan Nobile, Daniele Heguedusch, Giovanna Lopes Carvalho, Daniela Fátima Teixeira Silva, Rebeca Boltes Cecatto, Rodrigo Labat Marcos, Fabio Daumas Nunes, Maria Fernanda Setúbal Destro Rodrigues

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Abstract

This brief report aimed to investigate the optical absorbance spectra of normal, dysplastic, and malignant epithelial cell lines under normal and nutritional stress conditions. HaCAT (keratinocyte), DOK (oral dysplastic), and oral squamous cell carcinoma (OSCC) cell lines (CA1, Luc4, SCC9) were evaluated regarding their optical absorbance after culture with 0-10% fetal bovine serum. Absorbance measurements indicated that HaCAT under serum starvation exhibited higher absorbance at blue (430 nm) and near-infrared (906 nm) wavelengths. DOK showed absorption at 440 nm and 945 nm. OSCC cells showed absorption peaks at blue (400-428 nm) and near-infrared. These findings highlight the importance of tailoring PBM parameters to individual needs to achieve optimal absorption and effectiveness. Moreover, the higher absorption peaks in the blue region support further studies to elucidate the potential use of blue light in oral dysplastic lesions and OSCC.

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营养应激和血清饥饿对正常和恶性上皮细胞系光吸收的影响。

这篇简短的报告旨在研究正常、发育不良和恶性上皮细胞系在正常和营养胁迫条件下的光学吸收光谱。用0-10%胎牛血清培养后，对HaCAT（角化细胞）、DOK（口腔发育不良）和口腔鳞状细胞癌（OSCC）细胞系（CA1、Luc4、SCC9）的光学吸光度进行评估。吸光度测量表明，血清饥饿条件下HaCAT在蓝色（430 nm）和近红外（906 nm）波长处具有较高的吸光度。DOK在440 nm和945 nm处有吸收。OSCC细胞在蓝光（400 ~ 428 nm）和近红外波段出现吸收峰。这些发现强调了根据个体需要调整PBM参数以达到最佳吸收和效果的重要性。此外，蓝色区域较高的吸收峰支持进一步研究阐明蓝光在口腔发育不良病变和OSCC中的潜在应用。

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来源期刊

Lasers in Medical Science 医学-工程：生物医学

CiteScore

4.50

自引率

4.80%

发文量

192

审稿时长

3-8 weeks

期刊介绍： Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics. The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.