Photoinduced Chemiluminescence Determination of Prednisolone Using Lucigenin-Cobalt 5,10,15,20-Tetra(4-Pyridyl)-21H,23H-Porphine System

Abstract

Sensitive and precise assay of prednisolone, a steroid hormone, has received great attention due to its significant role in the treatment of a series of diseases. In this study, we have developed a simple, quick, and accurate technique in this part to measure prednisolone. Cobalt 5,10,15,20-tetra(4-pyridyl)-21H,23H-porphine (CoTPyP) as a mimic peroxidase catalyzes the chemiluminescence (CL) reaction of lucigenin for the first time. CoTPyP has a great impact on the CL signal. Furthermore, the CL system has been successfully used for the prednisolone flow-injection test. The findings showed that the UV irradiation of prednisolone led to an unimolecular photochemical reaction that could enhance yield of high-energy intermediate and cause higher CL emission. Under ideal experimental circumstances, CL intensity was shown to be proportionate to prednisolone concentration in the range of 0.1–170 μg mL⁻¹ (r = 0.9925, n = 4). A detection limit of 0.072 μg mL⁻¹ was observed. Compared with the HPLC approach, our proposed method provides a sensitive, fast, and efficient analysis of prednisolone in pharmaceutical formulations and biological fluids.