Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics.

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Metabolites Pub Date : 2025-01-16 DOI:10.3390/metabo15010060

Ekaterina Demicheva, Fernando Jonathan Polanco Espino, Pavel Vedeneev, Vadim Shevyrin, Aleksey Buhler, Elena Mukhlynina, Olga Berdiugina, Alicia Del Carmen Mondragon, Alberto Cepeda Sáez, Aroa Lopez-Santamarina, Alejandra Cardelle-Cobas, Olga Solovyova, Irina Danilova, Jose Manuel Miranda, Elena Kovaleva

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Abstract

Purpose: Immunodeficiency conditions, which are characterized by reduced immune activity that promotes the development of chronic diseases, are needed for efficient monitoring. A promising area of monitoring and early diagnosis of immunodeficiency diseases is the determination of metabolic biomarkers in the blood. Methods: In this work, we identified a set of lipid biomarkers of immunodeficiency states by performing high-performance liquid chromatography-high-resolution mass spectrometry (HPLC-HRMS) analysis of blood plasma samples from mice and processing them with bioinformatics approaches. Potential biomarkers were selected through statistical analysis and further validated by MS/MS. Conclusions: As a result, 15 lipids were confirmed and selected as potential biomarkers of immunodeficiency states. The selected biomarkers can be further studied and serve as promising targets for the early diagnosis of immunodeficiency diseases.

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环磷酰胺处理小鼠脂质谱的HPLC-HRMS和生物信息学比较研究。

目的：免疫缺陷状况的特点是免疫活性降低，从而促进慢性疾病的发展，因此需要对其进行有效监测。血液中代谢生物标志物的测定是免疫缺陷疾病监测和早期诊断的一个有前景的领域。方法：本研究通过高效液相色谱-高分辨率质谱（HPLC-HRMS）对小鼠血浆样本进行分析，并采用生物信息学方法对其进行处理，确定了一组免疫缺陷状态的脂质生物标志物。通过统计分析筛选出潜在的生物标志物，并用MS/MS进一步验证。结论：因此，15种脂质被确认并选择为免疫缺陷状态的潜在生物标志物。所选择的生物标志物可以进一步研究，并作为免疫缺陷疾病早期诊断的有希望的靶点。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.