{"title":"Generation of novel bpm6 and dmr6 mutants with broad-spectrum resistance using a modified CRISPR/Cas9 system in Brassica oleracea","authors":"Yulun Zhang, Jinhui Liu, Yingjie Li, Hongxue Ma, Jialei Ji, Yong Wang, Mu Zhuang, Limei Yang, Zhiyuan Fang, Jun Li, Chao Zhang, Liwang Liu, Marina Lebedeva, Vasiliy Taranov, Yangyong Zhang, Honghao Lv","doi":"10.1111/jipb.13842","DOIUrl":null,"url":null,"abstract":"<p>Using an optimized CRISPR/Cas9 system to knock out the BTB-POZ and MATH domain gene <i>BoBPM6</i> and the <i>DOWNY MILDEW RESISTANCE 6</i> gene in <i>Brassica oleracea</i> resulted in new lines with broad-spectrum disease resistance.\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 5","pages":"1214-1216"},"PeriodicalIF":9.3000,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13842","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13842","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Using an optimized CRISPR/Cas9 system to knock out the BTB-POZ and MATH domain gene BoBPM6 and the DOWNY MILDEW RESISTANCE 6 gene in Brassica oleracea resulted in new lines with broad-spectrum disease resistance.
期刊介绍：
Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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