Na Dong, Aiju Zhang, Xingde Dai, Xiaolin Zhang, Fangzhen He
{"title":"Determination of glucose oxidase activity of flour additive by fluorescence quenching method","authors":"Na Dong, Aiju Zhang, Xingde Dai, Xiaolin Zhang, Fangzhen He","doi":"10.1007/s11696-024-03848-x","DOIUrl":null,"url":null,"abstract":"<div><p>Glucose oxidase can catalyze the reaction of glucose to produce hydrogen peroxide, and hydrogen peroxide reacts with excess I<sup>−</sup> anions in dilute sulfuric acid solution to form I<sub>3</sub><sup>−</sup>. The I<sub>3</sub><sup>−</sup> combines with Rhodamine 6G to form association which result in fluorescence quenching. Effect of glucose oxidase on fluorescence of Rhodamine 6G, a new method with high sensitivity and good selectivity for the determination of glucose oxidase activity, was established. Under the optimal conditions, the linear range of the method is 1.0 × 10<sup>–4</sup>–1.0 × 10<sup>–1</sup> U/mL, and the detection limit is 9.0 × 10<sup>–5</sup> U/mL. This assay was applied to the determination of glucose oxidase activity in the food additives samples with satisfactory results; the recoveries obtained were 96.67–99.00%.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":513,"journal":{"name":"Chemical Papers","volume":"79 1","pages":"599 - 605"},"PeriodicalIF":2.5000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Papers","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s11696-024-03848-x","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Engineering","Score":null,"Total":0}
引用次数: 0
Abstract
Glucose oxidase can catalyze the reaction of glucose to produce hydrogen peroxide, and hydrogen peroxide reacts with excess I− anions in dilute sulfuric acid solution to form I3−. The I3− combines with Rhodamine 6G to form association which result in fluorescence quenching. Effect of glucose oxidase on fluorescence of Rhodamine 6G, a new method with high sensitivity and good selectivity for the determination of glucose oxidase activity, was established. Under the optimal conditions, the linear range of the method is 1.0 × 10–4–1.0 × 10–1 U/mL, and the detection limit is 9.0 × 10–5 U/mL. This assay was applied to the determination of glucose oxidase activity in the food additives samples with satisfactory results; the recoveries obtained were 96.67–99.00%.
Chemical PapersChemical Engineering-General Chemical Engineering
CiteScore
3.30
自引率
4.50%
发文量
590
期刊介绍：
Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.
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