Ibrahim Moshood , Małgorzata Maślanka , Anna Kapczyńska , Agnieszka Kiełkowska
{"title":"Isolation and culturing of protoplasts in endangered Lachenalia viridiflora","authors":"Ibrahim Moshood , Małgorzata Maślanka , Anna Kapczyńska , Agnieszka Kiełkowska","doi":"10.1016/j.sajb.2024.11.044","DOIUrl":null,"url":null,"abstract":"<div><div><em>Lachenalia viridiflora</em> is a critically endangered geophyte. This study aimed to develop a protocol for protoplast isolation and culture to enhance biotechnological tools for the improvement of this species. Protoplasts were isolated from micropropagated bulbils with leaves. Isolation yielded 1.2 × 10<sup>5</sup> to 1.7 × 10<sup>5</sup> protoplasts per gram of tissue, with 60–70 % viability. Protoplast-derived cells underwent divisions that resulted in microcallus formation and later shoot regeneration, demonstrating the potential of an elaborated protocol for successful protoplast-to-plant regeneration.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"177 ","pages":"Pages 88-91"},"PeriodicalIF":2.7000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"South African Journal of Botany","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254629924007762","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/5 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Lachenalia viridiflora is a critically endangered geophyte. This study aimed to develop a protocol for protoplast isolation and culture to enhance biotechnological tools for the improvement of this species. Protoplasts were isolated from micropropagated bulbils with leaves. Isolation yielded 1.2 × 105 to 1.7 × 105 protoplasts per gram of tissue, with 60–70 % viability. Protoplast-derived cells underwent divisions that resulted in microcallus formation and later shoot regeneration, demonstrating the potential of an elaborated protocol for successful protoplast-to-plant regeneration.
期刊介绍：
The South African Journal of Botany publishes original papers that deal with the classification, biodiversity, morphology, physiology, molecular biology, ecology, biotechnology, ethnobotany and other botanically related aspects of species that are of importance to southern Africa. Manuscripts dealing with significant new findings on other species of the world and general botanical principles will also be considered and are encouraged.
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