{"title":"Naked-eye sensor for rapid methamphetamine screening with analyte recovery","authors":"Seiya Awata , Futoshi Kikuchi , Toru Oba","doi":"10.1016/j.forc.2024.100634","DOIUrl":null,"url":null,"abstract":"<div><div>The feasibility of a simple and rapid test to detect trace amounts of methamphetamine (MA) based on host–guest molecular interactions is demonstrated. The macrocyclic host molecule, cucurbit[7]uril (CB[7]), encapsulates a single guest molecule of the indicator fluorescent dye, acridine orange (AO). Addition of MA to the mixture shifts the equilibrium by replacing the guest molecule, as reflected by the changes in AO fluorescence. Notably, the colors of 0 and 20 µM MA can be distinguished by the naked eye, and the sensitivity is 135 times higher than that of the conventional Simon’s reagent. Similar fluorescence changes have been observed in the AO•CB[7] assay between 15 and 3 °C in the pH range 6–8 in phosphate buffer. The CB[7] system can detect MA while excluding bulky amines and sugars. However, it is also sensitive to other MA-related compounds such as N-isopropylbenzylamine and dimethylsulfone. MA recovery from the host–guest complex solution was greater than 94 %, allowing more accurate analysis of valuable evidence after this rapid screening. These results indicate that the CB[7] supramolecular system for MA detection has the advantages of operational simplicity, rapidity, high sensitivity, and minimal sample consumption and is suitable for initial drug screening during field analysis.</div></div>","PeriodicalId":324,"journal":{"name":"Forensic Chemistry","volume":"42 ","pages":"Article 100634"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic Chemistry","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468170924000869","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/12 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
The feasibility of a simple and rapid test to detect trace amounts of methamphetamine (MA) based on host–guest molecular interactions is demonstrated. The macrocyclic host molecule, cucurbit[7]uril (CB[7]), encapsulates a single guest molecule of the indicator fluorescent dye, acridine orange (AO). Addition of MA to the mixture shifts the equilibrium by replacing the guest molecule, as reflected by the changes in AO fluorescence. Notably, the colors of 0 and 20 µM MA can be distinguished by the naked eye, and the sensitivity is 135 times higher than that of the conventional Simon’s reagent. Similar fluorescence changes have been observed in the AO•CB[7] assay between 15 and 3 °C in the pH range 6–8 in phosphate buffer. The CB[7] system can detect MA while excluding bulky amines and sugars. However, it is also sensitive to other MA-related compounds such as N-isopropylbenzylamine and dimethylsulfone. MA recovery from the host–guest complex solution was greater than 94 %, allowing more accurate analysis of valuable evidence after this rapid screening. These results indicate that the CB[7] supramolecular system for MA detection has the advantages of operational simplicity, rapidity, high sensitivity, and minimal sample consumption and is suitable for initial drug screening during field analysis.
期刊介绍：
Forensic Chemistry publishes high quality manuscripts focusing on the theory, research and application of any chemical science to forensic analysis. The scope of the journal includes fundamental advancements that result in a better understanding of the evidentiary significance derived from the physical and chemical analysis of materials. The scope of Forensic Chemistry will also include the application and or development of any molecular and atomic spectrochemical technique, electrochemical techniques, sensors, surface characterization techniques, mass spectrometry, nuclear magnetic resonance, chemometrics and statistics, and separation sciences (e.g. chromatography) that provide insight into the forensic analysis of materials. Evidential topics of interest to the journal include, but are not limited to, fingerprint analysis, drug analysis, ignitable liquid residue analysis, explosives detection and analysis, the characterization and comparison of trace evidence (glass, fibers, paints and polymers, tapes, soils and other materials), ink and paper analysis, gunshot residue analysis, synthetic pathways for drugs, toxicology and the analysis and chemistry associated with the components of fingermarks. The journal is particularly interested in receiving manuscripts that report advances in the forensic interpretation of chemical evidence. Technology Readiness Level: When submitting an article to Forensic Chemistry, all authors will be asked to self-assign a Technology Readiness Level (TRL) to their article. The purpose of the TRL system is to help readers understand the level of maturity of an idea or method, to help track the evolution of readiness of a given technique or method, and to help filter published articles by the expected ease of implementation in an operation setting within a crime lab.
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