Biotechnological valorization of yeast strains for lipids and carotenoids production from renewable resources

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biocatalysis and agricultural biotechnology Pub Date : 2025-02-01 Epub Date: 2025-01-20 DOI:10.1016/j.bcab.2025.103499

Andreísa Teixeira de Castro , Angélica Cristina de Souza , Cristina Ferreira Silva , Silvia Juliana Martinez , Rosane Freitas Schwan , Disney Ribeiro Dias

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Abstract

This study aimed to select yeast strains from the Agricultural Microbiology Culture Collection (CCMA) to produce lipids and/or carotenoids using crude glycerol and sugarcane molasses as alternative carbon sources. Among the selected strains, nine exhibited oleaginous characteristics, with Torulaspora maleeae (CCMA 0039) reaching the highest accumulation of lipid content (77.7%) in the glucose medium. Notably, the Exophiala spinifera (CCMA, 2073) strain exhibited 35% lipid accumulation in glycerol pure. Furthermore, Rhodotorula dairenensis (CCMA 945), Rhodotorula mucilaginosa (CCMA 0156), Rhodosporidium toruloides (CCMA, 2032) and Cystofilobasidium ferigula (CCMA 1623) were identified as carotenogenic. Crude glycerol has proven to be the most effective medium for lipogenesis and carotenogenesis, also expressing diversity in fatty acid profiles, with C. ferigula demonstrating promise in the coproduction of lipids and carotenoids, achieving 41.88% lipid accumulation and 2.76 μg/mL of total carotenoids. These findings highlight the potential for utilizing industrial by-products for the sustainable production of important metabolites, paving the way for their integration into industrial bioprocesses.

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利用可再生资源生产脂类和类胡萝卜素的酵母菌的生物技术鉴定

本研究旨在从农业微生物培养收集（CCMA）中选择酵母菌株，以粗甘油和甘蔗糖蜜作为替代碳源，生产脂质和/或类胡萝卜素。所选菌株中有9株具有产油特性，其中马来环孢菌（CCMA 0039）在葡萄糖培养基中脂含量积累最高，达到77.7%。值得注意的是，Exophiala spinifera （CCMA, 2073）菌株在甘油纯中表现出35%的脂质积累。此外，dairenrhodotorula （CCMA 945）、mucilaginosa （CCMA 0156）、toruloides Rhodosporidium （CCMA, 2032）和ferigula Cystofilobasidium （CCMA 1623）被鉴定为产胡萝卜素。粗甘油已被证明是脂肪生成和胡萝卜素生成最有效的培养基，在脂肪酸谱上也表现出多样性，而C. ferigula在脂质和类胡萝卜素的共同产生方面表现出了希望，脂质积累达到41.88%，类胡萝卜素总量达到2.76 μg/mL。这些发现强调了利用工业副产品可持续生产重要代谢物的潜力，为其融入工业生物过程铺平了道路。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.