Detection of AdeAB, TetA, and TetB efflux pump genes in clinical isolates of tetracycline-resistant Acinetobacter baumannii from patients of Suez Canal University Hospitals.

IF 4.2 2区生物学 Q2 MICROBIOLOGY BMC Microbiology Pub Date : 2025-02-04 DOI:10.1186/s12866-024-03735-1

Hasnaa Azab, Aya Mohamed Askar, Noha M Abd El-Fadeal, Amira A A Othman, Amal H Rayan, Sally Khattab

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Abstract

Background: Acinetobacter baumannii is an opportunistic bacteria associated primarily with hospital-acquired infections. Its tendency to acquire or donate resistance genes to neighboring bacteria is a major concern. Tetracyclines have shown promise in treating A. baumannii infections, but tetracycline resistance is growing globally in A. baumannii isolates.

Objectives: The study aimed to study (1) the prevalence of multidrug-resistant (MDR) A. baumannii infections at Suez Canal University Hospitals, (2) the distribution of efflux pump genes AdeA &B, TetA, and TetB, and (3) the effect of efflux pump inhibitor (CCCP) on tetracycline-resistant isolates.

Methods: Clinical samples (457) were collected (blood, urine, sputum, ETA, pus, and pleural fluid), followed by A. baumannii isolation and identification, PCR detection of efflux pump genes, and detection of tetracycline susceptibility and its MIC before and after treatment with the efflux pump inhibitor (CCCP).

Results: A total of 31 A. baumannii isolates were recovered (6.78%). The highest rate of isolation was from the ICU (48.3%) from the ET aspirate samples (48.3%). The efflux system AdeA and TetB genes were distributed in 100% of isolates, whereas AdeB was found in 93.5% of isolates and the TetA gene in 87.1% of isolates. All A. baumannii isolates were MDR showing resistance to three or more classes of antibiotics. 45% of the isolates showed a 4-fold reduction of MIC and 12.9% showed a 2-fold reduction in the MIC.

Conclusions: Efflux pump is an important mechanism for tetracycline resistance among A. baumannii isolates.

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检测苏伊士运河大学医院患者临床分离的耐四环素鲍曼不动杆菌中的 AdeAB、TetA 和 TetB 外排泵基因。

背景：鲍曼不动杆菌是一种主要与医院获得性感染相关的机会性细菌。它倾向于获得或将抗性基因捐赠给邻近的细菌是一个主要问题。四环素已显示出治疗鲍曼不动杆菌感染的希望，但鲍曼不动杆菌分离株对四环素的耐药性正在全球范围内增长。目的：研究(1)苏伊士运河大学医院多药耐药（MDR）鲍曼不动杆菌感染的流行情况；(2)外排泵基因AdeA &B、TetA和TetB的分布情况；(3)外排泵抑制剂（CCCP）对四环素耐药菌株的影响。方法：收集临床标本457例（血、尿、痰、ETA、脓、胸膜液），分离鉴定鲍曼不动杆菌，检测外排泵基因PCR检测，检测外排泵抑制剂（CCCP）治疗前后四环素敏感性及MIC。结果：共检出鲍曼不动杆菌31株（6.78%）。分离率最高的是ICU（48.3%）和ET抽吸标本（48.3%）。外排系统AdeA和TetB基因分布在100%的分离株中，而AdeB基因分布在93.5%的分离株中，TetA基因分布在87.1%的分离株中。所有鲍曼不动杆菌分离株均为耐多药，对三种或三种以上抗生素具有耐药性。45%的分离株MIC降低了4倍，12.9%的分离株MIC降低了2倍。结论：外排泵是鲍曼不动杆菌产生四环素耐药的重要机制。

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来源期刊

BMC Microbiology 生物-微生物学

CiteScore

7.20

自引率

0.00%

发文量

280

审稿时长

3 months

期刊介绍： BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.