Protocol for measuring the auxin-induced changes of m6A modification.

Abstract

Plant m⁶A modification is highly dynamic, with plant hormones serving as key regulators that induce these dynamics. Here, we present a protocol for detecting phytohormone-induced changes in m⁶A modification using RNA dot blot and m⁶A sequencing (m⁶A-seq) techniques. We describe steps for the auxin treatment, RNA dot blot and m⁶A-seq library preparation, and data analysis. This protocol holds potential applications for analyzing changes in m⁶A modification induced by plant hormones. For complete details on the use and execution of this protocol, please refer to Bin et al.¹.