{"title":"Determination of phenolic constituents with LC-MS/MS and cytotoxicity evaluation of 'tar' from <i>Pinus nigra</i> Arnold <i>(Pinaceae)</i>.","authors":"Arzu Özgen","doi":"10.1080/14786419.2025.2463118","DOIUrl":null,"url":null,"abstract":"<p><p>In folk medicine, 'tar' is an essential drug in every situation and is obtained from <i>Pinus nigra</i> Arnold <i>(Pinaceae)</i>, which is very common in Anatolia, especially in the Mediterranean Basin. This study aims to evaluate the chemical composition and determine cytotoxicity effects on the HeLa cell line and L929 cell line of the tar. The analysis of tar by LC-MS/MS revealed ten phenolic compounds: oxalic acid, salicylic acid, vanillic acid, ferulic acid, caffeic acid, 4-hydroxybenzoic acid, cinnamic acid, <i>p</i>-coumaric acid, and 3-hydroxycinnamic acid. Besides, total phenolic content (62.31 ± 0.31 mg GAE/g) and flavonoid content (3.43 ± 0.0006 mg QE/g) were determined. Cell viability was determined by MTT analysis. Tar has a significant cytotoxicity effect on the HeLa cell line in concentrations 100, 50, 25, 12.5, and 6.25 µg/mL. IC50 value of tar on HeLa cell was 132.9 µg/mL.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.6000,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/14786419.2025.2463118","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
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Abstract
In folk medicine, 'tar' is an essential drug in every situation and is obtained from Pinus nigra Arnold (Pinaceae), which is very common in Anatolia, especially in the Mediterranean Basin. This study aims to evaluate the chemical composition and determine cytotoxicity effects on the HeLa cell line and L929 cell line of the tar. The analysis of tar by LC-MS/MS revealed ten phenolic compounds: oxalic acid, salicylic acid, vanillic acid, ferulic acid, caffeic acid, 4-hydroxybenzoic acid, cinnamic acid, p-coumaric acid, and 3-hydroxycinnamic acid. Besides, total phenolic content (62.31 ± 0.31 mg GAE/g) and flavonoid content (3.43 ± 0.0006 mg QE/g) were determined. Cell viability was determined by MTT analysis. Tar has a significant cytotoxicity effect on the HeLa cell line in concentrations 100, 50, 25, 12.5, and 6.25 µg/mL. IC50 value of tar on HeLa cell was 132.9 µg/mL.
期刊介绍：
The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds.
The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal.
Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section.
All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.
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